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SINGLE: Atomic-resolution structure identification of nanocrystals by graphene liquid cell EM

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Title
SINGLE: Atomic-resolution structure identification of nanocrystals by graphene liquid cell EM
Author(s)
Reboul, Cyril F.; Junyoung Heo; Machello, Chiara; Kiesewetter, Simon; Byung Hyo Kim; Sungin Kim; Elmlund, Dominika; Ercius, Peter; Jungwon Park; Elmlund, Hans
Publication Date
2021-01
Journal
Science Advances, v.7, no.5
Publisher
American Association for the Advancement of Science
Abstract
Copyright © 2021 The Authors, some rights reserved;Analysis of the three-dimensional (3D) structures of nanocrystals with solution-phase transmission electron microscopy is beginning to reveal their unique physiochemical properties. We developed a “one-particle Brownian 3D reconstruction method” based on imaging of ensembles of colloidal nanocrystals using graphene liquid cell electron microscopy. Projection images of differently rotated nanocrystals are acquired using a direct electron detector with high temporal (<2.5 ms) resolution and analyzed to obtain an ensemble of 3D reconstructions. Here, we introduce computational methods required for successful atomic-resolution 3D reconstruction: (i) tracking of the individual particles throughout the time series, (ii) subtraction of the interfering background of the graphene liquid cell, (iii) identification and rejection of low-quality images, and (iv) tailored strategies for 2D/3D alignment and averaging that differ from those used in biological cryo–electron microscopy. Our developments are made available through the open-source software package SINGLE.
URI
https://pr.ibs.re.kr/handle/8788114/9864
DOI
10.1126/SCIADV.ABE6679
ISSN
2375-2548
Appears in Collections:
Center for Nanoparticle Research(나노입자 연구단) > 1. Journal Papers (저널논문)
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