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SINGLE: Atomic-resolution structure identification of nanocrystals by graphene liquid cell EM

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dc.contributor.authorReboul, Cyril F.-
dc.contributor.authorJunyoung Heo-
dc.contributor.authorMachello, Chiara-
dc.contributor.authorKiesewetter, Simon-
dc.contributor.authorByung Hyo Kim-
dc.contributor.authorSungin Kim-
dc.contributor.authorElmlund, Dominika-
dc.contributor.authorErcius, Peter-
dc.contributor.authorJungwon Park-
dc.contributor.authorElmlund, Hans-
dc.date.accessioned2021-07-07T02:30:07Z-
dc.date.accessioned2021-07-07T02:30:07Z-
dc.date.available2021-07-07T02:30:07Z-
dc.date.available2021-07-07T02:30:07Z-
dc.date.created2021-03-09-
dc.date.issued2021-01-
dc.identifier.issn2375-2548-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/9864-
dc.description.abstractCopyright © 2021 The Authors, some rights reserved;Analysis of the three-dimensional (3D) structures of nanocrystals with solution-phase transmission electron microscopy is beginning to reveal their unique physiochemical properties. We developed a “one-particle Brownian 3D reconstruction method” based on imaging of ensembles of colloidal nanocrystals using graphene liquid cell electron microscopy. Projection images of differently rotated nanocrystals are acquired using a direct electron detector with high temporal (<2.5 ms) resolution and analyzed to obtain an ensemble of 3D reconstructions. Here, we introduce computational methods required for successful atomic-resolution 3D reconstruction: (i) tracking of the individual particles throughout the time series, (ii) subtraction of the interfering background of the graphene liquid cell, (iii) identification and rejection of low-quality images, and (iv) tailored strategies for 2D/3D alignment and averaging that differ from those used in biological cryo–electron microscopy. Our developments are made available through the open-source software package SINGLE.-
dc.description.uri1-
dc.language영어-
dc.publisherAmerican Association for the Advancement of Science-
dc.titleSINGLE: Atomic-resolution structure identification of nanocrystals by graphene liquid cell EM-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000614004600046-
dc.identifier.scopusid2-s2.0-85100649606-
dc.identifier.rimsid74905-
dc.contributor.affiliatedAuthorJunyoung Heo-
dc.contributor.affiliatedAuthorByung Hyo Kim-
dc.contributor.affiliatedAuthorSungin Kim-
dc.contributor.affiliatedAuthorJungwon Park-
dc.identifier.doi10.1126/SCIADV.ABE6679-
dc.identifier.bibliographicCitationScience Advances, v.7, no.5-
dc.citation.titleScience Advances-
dc.citation.volume7-
dc.citation.number5-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalWebOfScienceCategoryMultidisciplinary Sciences-
Appears in Collections:
Center for Nanoparticle Research(나노입자 연구단) > 1. Journal Papers (저널논문)
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