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Chang, Young Tae
복잡계 자기조립 연구단
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Direct monitoring of live human pluripotent stem cells by a highly selective pluripotency sensor

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Title
Direct monitoring of live human pluripotent stem cells by a highly selective pluripotency sensor
Author(s)
Myung Rae Park; Donggyu Nam; Hyunah Lee; Young-Tae Chang; Holm Zaehres; Jeong Beom Kim
Subject
BODIPY, ; DOFLA, ; Human pluripotent stem cells, ; Pluripotency, ; Sensor
Publication Date
2020-08
Journal
Bioorganic and Medicinal Chemistry Letters, v.30, no.16, pp.127347
Publisher
ELSEVIER
Abstract
© 2020 Elsevier Ltd. Human pluripotent stem cells (hPSCs) are a useful cell source for regenerative medicine. Despite having a potential of hPSCs for cell-based therapy, there is a need for a selective human pluripotency sensor for monitoring of live hPSCs. Here, we report the discovery of a novel pluripotency sensor (SHI5) from BODIPY-based library by high-throughput cell-based screening and describe the use of SHI5 to identify and isolate human embryonic stem cells and human induced pluripotent stem cells. We demonstrate that SHI5-based assay can be applied to live cells that gain pluripotency in the reprogramming process without any effect on their viability. We also show that SHI5 is internalized through a clathrin-mediated endocytosis pathway. These findings suggest that SHI5 can be an attractive sensor for pluripotency cells during reprogramming. Taken together, SHI5-based screening for hPSCs opens probably unlimited possibilities of detection probe for hPSC therapy via assures their safety issue
URI
https://pr.ibs.re.kr/handle/8788114/7713
DOI
10.1016/j.bmcl.2020.127347
ISSN
0960-894X
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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