Direct monitoring of live human pluripotent stem cells by a highly selective pluripotency sensor
DC Field | Value | Language |
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dc.contributor.author | Myung Rae Park | - |
dc.contributor.author | Donggyu Nam | - |
dc.contributor.author | Hyunah Lee | - |
dc.contributor.author | Young-Tae Chang | - |
dc.contributor.author | Holm Zaehres | - |
dc.contributor.author | Jeong Beom Kim | - |
dc.date.accessioned | 2020-12-22T02:49:44Z | - |
dc.date.accessioned | 2020-12-22T02:49:44Z | - |
dc.date.available | 2020-12-22T02:49:44Z | - |
dc.date.available | 2020-12-22T02:49:44Z | - |
dc.date.created | 2020-07-22 | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 0960-894X | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/7713 | - |
dc.description.abstract | © 2020 Elsevier Ltd. Human pluripotent stem cells (hPSCs) are a useful cell source for regenerative medicine. Despite having a potential of hPSCs for cell-based therapy, there is a need for a selective human pluripotency sensor for monitoring of live hPSCs. Here, we report the discovery of a novel pluripotency sensor (SHI5) from BODIPY-based library by high-throughput cell-based screening and describe the use of SHI5 to identify and isolate human embryonic stem cells and human induced pluripotent stem cells. We demonstrate that SHI5-based assay can be applied to live cells that gain pluripotency in the reprogramming process without any effect on their viability. We also show that SHI5 is internalized through a clathrin-mediated endocytosis pathway. These findings suggest that SHI5 can be an attractive sensor for pluripotency cells during reprogramming. Taken together, SHI5-based screening for hPSCs opens probably unlimited possibilities of detection probe for hPSC therapy via assures their safety issue | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER | - |
dc.subject | BODIPY | - |
dc.subject | DOFLA | - |
dc.subject | Human pluripotent stem cells | - |
dc.subject | Pluripotency | - |
dc.subject | Sensor | - |
dc.title | Direct monitoring of live human pluripotent stem cells by a highly selective pluripotency sensor | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000551073500011 | - |
dc.identifier.scopusid | 2-s2.0-85086999128 | - |
dc.identifier.rimsid | 72628 | - |
dc.contributor.affiliatedAuthor | Young-Tae Chang | - |
dc.identifier.doi | 10.1016/j.bmcl.2020.127347 | - |
dc.identifier.bibliographicCitation | Bioorganic and Medicinal Chemistry Letters, v.30, no.16, pp.127347 | - |
dc.citation.title | Bioorganic and Medicinal Chemistry Letters | - |
dc.citation.volume | 30 | - |
dc.citation.number | 16 | - |
dc.citation.startPage | 127347 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | BODIPY | - |
dc.subject.keywordAuthor | DOFLA | - |
dc.subject.keywordAuthor | Human pluripotent stem cells | - |
dc.subject.keywordAuthor | Pluripotency | - |
dc.subject.keywordAuthor | Sensor | - |