BROWSE

Related Scientist

Researcher

지아영
첨단연성물질 연구단
more info

Enhanced Diffusion and Oligomeric Enzyme Dissociation

Cited 0 time in webofscience Cited 0 time in scopus
33 Viewed 6 Downloaded
Title
Enhanced Diffusion and Oligomeric Enzyme Dissociation
Author(s)
Ah-Young Jee; Kuo Chen; Tsvi Tlusty; Jiang Zhao; Steve Granick
Publication Date
2019-12
Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.141, no.51, pp.20068 -
Publisher
AMER CHEMICAL SOC
Abstract
© 2019 American Chemical Society.The concept that catalytic enzymes can act as molecular machines transducing chemical activity into motion has conceptual and experimental support, but experimental support has involved oligomeric enzymes, often studied under conditions where the substrate concentration is higher than biologically relevant and accordingly exceeds kM, the Michaelis constant. Urease, a hexamer of subunits, has been considered to be the gold standard demonstrating enhanced diffusion. Here we show that urease and certain other oligomeric enzymes dissociate above kM into their subunits that diffuse more rapidly, thus providing a simple physical mechanism that contributes to enhanced diffusion in this regime of concentrations. Mindful that this conclusion may be controversial, our findings are supported by four independent analytical techniques: static light scattering, dynamic light scattering (DLS), size-exclusion chromatography (SEC), and fluorescence correlation spectroscopy (FCS). Data for urease are emphasized and the conclusion is validated for hexokinase, acetylcholinesterase, and aldolase. For hexokinase and aldolase no enhanced diffusion is observed except under conditions when these oligomeric enzymes dissociate. At substrate concentration regimes below kM at which acetylcholinesterase and urease do not dissociate, our finding showing up to 10% enhancement of the diffusion coefficient is consistent with various theoretical scenarios in the literature
URI
https://pr.ibs.re.kr/handle/8788114/6746
ISSN
0002-7863
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
Files in This Item:
2019_JACS_Steve_Enhanced Diffusion and Oligomeric Enzyme Dissociation.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse