Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate

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Title
Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate
Author(s)
Beomsue Kim; Masahiro Fukuda; Jung-Yeol Lee; Dongdong Su; Srikanta Sanu; Aymeric Silvin; Audrey T. T. Khoo; Taejoon Kwon; Xiao Liu; Weijie Chi; Xiaogang Liu; Sejong Choi; Diana S. Y. Wan; Sung-Jin Park; Jin Soo Kim; Florent Ginhoux; H. Shawn Je; Young Tae Chang
Publication Date
2019-06
Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v.58, no.24, pp.7972 - 7976
Publisher
WILEY-V C H VERLAG GMBH
Abstract
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure–activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence
URI
https://pr.ibs.re.kr/handle/8788114/6068
ISSN
1433-7851
Appears in Collections:
Center for Genome Engineering(유전체 교정 연구단) > Journal Papers (저널논문)
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
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