Efficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins

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Title
Efficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins
Author(s)
Lee, E; Min, K; Chang, YT; Kwon, Y
Publication Date
2018-09
Journal
PROTEIN SCIENCE, v.27, no.9, pp.1568 - 1574
Publisher
WILEY
Abstract
An efficient and wash-free method to conjugate a fluorescent tag to a target membrane protein is developed, using engineered Npu DnaE split-inteins. This approach allowed fast labeling while avoiding the strenuous synthesis of a long polypeptide. Two different modes of labeling, namely specific binding and covalent conjugation, are observed. The covalent labeling was monitored within 5 min, without background staining. Published by Wiley © 2018 The Protein Society
URI
https://pr.ibs.re.kr/handle/8788114/5779
ISSN
0961-8368
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
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