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복잡계자기조립연구단
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Efficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins

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dc.contributor.authorLee, E-
dc.contributor.authorMin, K-
dc.contributor.authorChang, YT-
dc.contributor.authorKwon, Y-
dc.date.available2019-05-02T08:10:34Z-
dc.date.created2019-03-13-
dc.date.issued2018-09-
dc.identifier.issn0961-8368-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/5779-
dc.description.abstractAn efficient and wash-free method to conjugate a fluorescent tag to a target membrane protein is developed, using engineered Npu DnaE split-inteins. This approach allowed fast labeling while avoiding the strenuous synthesis of a long polypeptide. Two different modes of labeling, namely specific binding and covalent conjugation, are observed. The covalent labeling was monitored within 5 min, without background staining. Published by Wiley © 2018 The Protein Society-
dc.description.uri1-
dc.language영어-
dc.publisherWILEY-
dc.subjectsplit-intein-
dc.subjectprotein labeling-
dc.subjectprotein trans-splicing-
dc.subjectprotein engineering-
dc.titleEfficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000447779000003-
dc.identifier.scopusid2-s2.0-85054013645-
dc.identifier.rimsid67434-
dc.contributor.affiliatedAuthorChang, YT-
dc.identifier.doi10.1002/pro.3455-
dc.identifier.bibliographicCitationPROTEIN SCIENCE, v.27, no.9, pp.1568 - 1574-
dc.citation.titlePROTEIN SCIENCE-
dc.citation.volume27-
dc.citation.number9-
dc.citation.startPage1568-
dc.citation.endPage1574-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusCELL-SURFACE PROTEINS-
dc.subject.keywordPlusNOSTOC-PUNCTIFORME-
dc.subject.keywordPlusLIVE CELLS-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusACTIVATION-
dc.subject.keywordPlusBIOSENSOR-
dc.subject.keywordPlusDESIGN-
dc.subject.keywordPlusTAGS-
dc.subject.keywordAuthorsplit-intein-
dc.subject.keywordAuthorprotein labeling-
dc.subject.keywordAuthorprotein trans-splicing-
dc.subject.keywordAuthorprotein engineering-
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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