Efficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, E | - |
dc.contributor.author | Min, K | - |
dc.contributor.author | Chang, YT | - |
dc.contributor.author | Kwon, Y | - |
dc.date.available | 2019-05-02T08:10:34Z | - |
dc.date.created | 2019-03-13 | - |
dc.date.issued | 2018-09 | - |
dc.identifier.issn | 0961-8368 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/5779 | - |
dc.description.abstract | An efficient and wash-free method to conjugate a fluorescent tag to a target membrane protein is developed, using engineered Npu DnaE split-inteins. This approach allowed fast labeling while avoiding the strenuous synthesis of a long polypeptide. Two different modes of labeling, namely specific binding and covalent conjugation, are observed. The covalent labeling was monitored within 5 min, without background staining. Published by Wiley © 2018 The Protein Society | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | WILEY | - |
dc.subject | split-intein | - |
dc.subject | protein labeling | - |
dc.subject | protein trans-splicing | - |
dc.subject | protein engineering | - |
dc.title | Efficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000447779000003 | - |
dc.identifier.scopusid | 2-s2.0-85054013645 | - |
dc.identifier.rimsid | 67434 | - |
dc.contributor.affiliatedAuthor | Chang, YT | - |
dc.identifier.doi | 10.1002/pro.3455 | - |
dc.identifier.bibliographicCitation | PROTEIN SCIENCE, v.27, no.9, pp.1568 - 1574 | - |
dc.citation.title | PROTEIN SCIENCE | - |
dc.citation.volume | 27 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1568 | - |
dc.citation.endPage | 1574 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | CELL-SURFACE PROTEINS | - |
dc.subject.keywordPlus | NOSTOC-PUNCTIFORME | - |
dc.subject.keywordPlus | LIVE CELLS | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | TAGS | - |
dc.subject.keywordAuthor | split-intein | - |
dc.subject.keywordAuthor | protein labeling | - |
dc.subject.keywordAuthor | protein trans-splicing | - |
dc.subject.keywordAuthor | protein engineering | - |