BROWSE

Related Scientist

jee,ahyoung's photo.

jee,ahyoung
인공지능및로봇기반합성연구단
more info

ITEM VIEW & DOWNLOAD

DNA molecules deviate from shortest trajectory when driven through hydrogel

Cited 4 time in webofscience Cited 3 time in scopus
828 Viewed 209 Downloaded
Title
DNA molecules deviate from shortest trajectory when driven through hydrogel
Author(s)
Juan Guan; Kejia Chen; Ah-Young Jee; Steve Granick
Publication Date
2018-10
Journal
JOURNAL OF CHEMICAL PHYSICS, v.149, no.16, pp.163331-1 - 163331-6
Publisher
AMER INST PHYSICS
Abstract
Dynamic fluorescence-based single-molecule imaging of λ-DNA molecules driven through agarose hydrogels by DC electric fields reveals that passage through the hydrogel (98.5% water content) induces mobility orthogonal to the external field. Tortuous paths followed by the DNA molecules, which are heavily entangled in the hydrogel mesh as their contour length is nearly 100 times the hydrogel mesh size of 200 nm, cause them to appear to diffuse orthogonal to the driving force. The higher the driving field, from 2 to 16 V/cm, the higher the off-axis dispersion is, over the same time interval. We measure the off-axis displacement distribution over 3 orders of magnitude of probability density and find a master curve after normalizing for time (t) elapsed, but the power of time for normalizing increases with the external field, from t0.25 to t0.6 with increasing field. Comparing trajectories over the same distance traveled in the electric field direction, we observe whereas for the highest field strengths DNA molecules come closest to taking the shortest trajectory between two points in space, deviations from the shortest trajectory grow larger and larger (up to 40% larger) as one approaches the case of small yet finite external field strength. © 2018 Author(s)
URI
https://pr.ibs.re.kr/handle/8788114/5145
DOI
10.1063/1.5033990
ISSN
0021-9606
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
Granick_Journal of Chemical Physics.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse