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DNA molecules deviate from shortest trajectory when driven through hydrogel

DC Field Value Language
dc.contributor.authorJuan Guan-
dc.contributor.authorKejia Chen-
dc.contributor.authorAh-Young Jee-
dc.contributor.authorSteve Granick-
dc.date.available2019-01-03T05:32:01Z-
dc.date.created2018-09-17-
dc.date.issued2018-10-
dc.identifier.issn0021-9606-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/5145-
dc.description.abstractDynamic fluorescence-based single-molecule imaging of λ-DNA molecules driven through agarose hydrogels by DC electric fields reveals that passage through the hydrogel (98.5% water content) induces mobility orthogonal to the external field. Tortuous paths followed by the DNA molecules, which are heavily entangled in the hydrogel mesh as their contour length is nearly 100 times the hydrogel mesh size of 200 nm, cause them to appear to diffuse orthogonal to the driving force. The higher the driving field, from 2 to 16 V/cm, the higher the off-axis dispersion is, over the same time interval. We measure the off-axis displacement distribution over 3 orders of magnitude of probability density and find a master curve after normalizing for time (t) elapsed, but the power of time for normalizing increases with the external field, from t0.25 to t0.6 with increasing field. Comparing trajectories over the same distance traveled in the electric field direction, we observe whereas for the highest field strengths DNA molecules come closest to taking the shortest trajectory between two points in space, deviations from the shortest trajectory grow larger and larger (up to 40% larger) as one approaches the case of small yet finite external field strength. © 2018 Author(s)-
dc.description.uri1-
dc.language영어-
dc.publisherAMER INST PHYSICS-
dc.titleDNA molecules deviate from shortest trajectory when driven through hydrogel-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000449103200033-
dc.identifier.scopusid2-s2.0-85051343155-
dc.identifier.rimsid65466-
dc.contributor.affiliatedAuthorAh-Young Jee-
dc.contributor.affiliatedAuthorSteve Granick-
dc.identifier.doi10.1063/1.5033990-
dc.identifier.bibliographicCitationJOURNAL OF CHEMICAL PHYSICS, v.149, no.16, pp.163331-1 - 163331-6-
dc.citation.titleJOURNAL OF CHEMICAL PHYSICS-
dc.citation.volume149-
dc.citation.number16-
dc.citation.startPage163331-1-
dc.citation.endPage163331-6-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusCOLLOIDAL GLASS-TRANSITION-
dc.subject.keywordPlusDISPERSION COEFFICIENTS-
dc.subject.keywordPlusGEL-ELECTROPHORESIS-
dc.subject.keywordPlusAGAROSE GELS-
dc.subject.keywordPlusTRANSVERSE DISPERSION-
dc.subject.keywordPlusBACTERIAL CHEMOTAXIS-
dc.subject.keywordPlusDIFFUSION-
dc.subject.keywordPlusTRANSPORT-
dc.subject.keywordPlusMACROMOLECULES-
dc.subject.keywordPlusSUSPENSIONS-
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > 1. Journal Papers (저널논문)
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