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Preparation of Homogeneous Solid Samples for Reproducible and Quantitative MALDI

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Title
Preparation of Homogeneous Solid Samples for Reproducible and Quantitative MALDI
Author(s)
Ahn, SH; Park, KM; Moon, JH; Lee, SH; Myung Soo Kim
Publication Date
2016-04
Journal
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.37, no.4, pp.458 - 462
Publisher
KOREAN CHEMICAL SOC
Abstract
Preparing samples with good homogeneity is one of the requirements for generating reproducible matrix-assisted laser desorption ionization (MALDI) spectra and for using them in analyte quantification. Thus far, we have not been successful in producing homogeneous solid samples from popular matrixes apart from -cyano-4-hydroxycinnamic acid (CHCA). In this work, we demonstrate the production of an outwardly homogeneous solid sample by loading a methanol solution of a matrix into a shallow reservoir on a coated MALDI sample plate and then vacuum-drying it. Out of ten popular matrixes tested, seven yielded homogeneous samples. These were 9-aminoacridine, 6-aza-2-thiothymine, CHCA, 2,5-dihydroxybenzoic acid, ferulic acid, sinapinic acid, and 2,4,6-trihydroxyacetophenone. For MALDI with these matrixes, linear calibration curves covering wide dynamic ranges were acquired. Performances of these matrixes in various aspects of analyte quantification have been investigated. © 2016 Korean Chemical Society, Seoul & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
URI
https://pr.ibs.re.kr/handle/8788114/3173
ISSN
0253-2964
Appears in Collections:
Center for Nanoparticle Research(나노입자 연구단) > Journal Papers (저널논문)
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