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Preparation of Homogeneous Solid Samples for Reproducible and Quantitative MALDI

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dc.contributor.authorAhn, SH-
dc.contributor.authorPark, KM-
dc.contributor.authorMoon, JH-
dc.contributor.authorLee, SH-
dc.contributor.authorMyung Soo Kim-
dc.date.available2017-01-06T02:05:14Z-
dc.date.created2016-05-17-
dc.date.issued2016-04-
dc.identifier.issn0253-2964-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/3173-
dc.description.abstractPreparing samples with good homogeneity is one of the requirements for generating reproducible matrix-assisted laser desorption ionization (MALDI) spectra and for using them in analyte quantification. Thus far, we have not been successful in producing homogeneous solid samples from popular matrixes apart from -cyano-4-hydroxycinnamic acid (CHCA). In this work, we demonstrate the production of an outwardly homogeneous solid sample by loading a methanol solution of a matrix into a shallow reservoir on a coated MALDI sample plate and then vacuum-drying it. Out of ten popular matrixes tested, seven yielded homogeneous samples. These were 9-aminoacridine, 6-aza-2-thiothymine, CHCA, 2,5-dihydroxybenzoic acid, ferulic acid, sinapinic acid, and 2,4,6-trihydroxyacetophenone. For MALDI with these matrixes, linear calibration curves covering wide dynamic ranges were acquired. Performances of these matrixes in various aspects of analyte quantification have been investigated. © 2016 Korean Chemical Society, Seoul & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim-
dc.description.uri1-
dc.language영어-
dc.publisherKOREAN CHEMICAL SOC-
dc.subjectMatrix-assisted laser desorption ionization-
dc.subjectMALDI quantification-
dc.subjectReproducible MALDI-
dc.subjectMALDI temperature-
dc.subjectMatrix suppression-
dc.titlePreparation of Homogeneous Solid Samples for Reproducible and Quantitative MALDI-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000374009600007-
dc.identifier.scopusid2-s2.0-84960153579-
dc.identifier.rimsid55369ko
dc.date.tcdate2018-10-01-
dc.contributor.affiliatedAuthorMyung Soo Kim-
dc.identifier.doi10.1002/bkcs.10704-
dc.identifier.bibliographicCitationBULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.37, no.4, pp.458 - 462-
dc.citation.titleBULLETIN OF THE KOREAN CHEMICAL SOCIETY-
dc.citation.volume37-
dc.citation.number4-
dc.citation.startPage458-
dc.citation.endPage462-
dc.date.scptcdate2018-10-01-
dc.description.wostc4-
dc.description.scptc4-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
dc.subject.keywordPlusLASER-DESORPTION IONIZATION-
dc.subject.keywordPlusDESORPTION/IONIZATION MASS-SPECTROMETRY-
dc.subject.keywordPlusIONIC-LIQUID MATRICES-
dc.subject.keywordPlusTOF-MS-
dc.subject.keywordPlusPEPTIDES-
dc.subject.keywordPlusSPECTRA-
dc.subject.keywordPlusQUANTIFICATION-
dc.subject.keywordPlusFRAGMENTATION-
dc.subject.keywordPlusTEMPERATURE-
dc.subject.keywordPlusPROTEINS-
dc.subject.keywordAuthorMatrix-assisted laser desorption ionization-
dc.subject.keywordAuthorMALDI quantification-
dc.subject.keywordAuthorReproducible MALDI-
dc.subject.keywordAuthorMALDI temperature-
dc.subject.keywordAuthorMatrix suppression-
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Center for Nanoparticle Research(나노입자 연구단) > 1. Journal Papers (저널논문)
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