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복잡계 자기조립 연구단
복잡계 자기조립 연구단
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An approach toward SNP detection by modulating the fluorescence of DNA-templated silver nanoclusters

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Title
An approach toward SNP detection by modulating the fluorescence of DNA-templated silver nanoclusters
Author(s)
Juhee Park; Jihyun Lee; Ban, Changill; Won Jong Kim
Publication Date
2013-05
Journal
BIOSENSORS & BIOELECTRONICS, v.43, no.1, pp.419 - 424
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Abstract
A rapid,homogeneous,andin-situlabelableformatdesignedtodetectsinglenucleotidepolymorphism (SNP) ispresented.Fluorescentsilvernanoclustersproducedfollowinghydride-mediatedreductionof Agþ boundtoapartialdouble-strandedoligodeoxynucleotidewereemployedasprobesforSNPs.The sensingmechanismisbasedonthefluorescenceenhancementofsilvernanoclustersthroughan irreversibleclustertransferuponhybridization.Thekeyelementofourdesignismodulatingthe‘‘turn on’’ mechanismbyintroducingacompetitorthatcanbedisplacedinresponsetoatargetDNA.Ina controlledmodelsystem,thefluorescenceintensityofsilvernanoclustersisapproximately3-fold enhanceduponhybridizationwithaperfectlymatchedtargetDNA,andsingle-basemismatch detectionisachievedwithin5min,regardlessofthepositionofmismatches.Humanaldehyde dehydrogenase2(ALDH2),whichisresponsiblefortheoxidationofaldehydestocarboxylicacids,is then utilizedasatargetforSNP.Uponadditionofaperfectlymatchedsequence,dramaticallyenhanced fluorescenceintensityisobserved(ca.48-fold),andpromptSNPgenotypingisaccomplished.
URI
https://pr.ibs.re.kr/handle/8788114/1335
ISSN
0956-5663
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
Files in This Item:
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