An approach toward SNP detection by modulating the fluorescence of DNA-templated silver nanoclusters
DC Field | Value | Language |
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dc.contributor.author | Juhee Park | - |
dc.contributor.author | Jihyun Lee | - |
dc.contributor.author | Ban, Changill | - |
dc.contributor.author | Won Jong Kim | - |
dc.date.available | 2015-04-20T07:01:50Z | - |
dc.date.created | 2014-08-11 | - |
dc.date.issued | 2013-05 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/1335 | - |
dc.description.abstract | A rapid,homogeneous,andin-situlabelableformatdesignedtodetectsinglenucleotidepolymorphism (SNP) ispresented.Fluorescentsilvernanoclustersproducedfollowinghydride-mediatedreductionof Agþ boundtoapartialdouble-strandedoligodeoxynucleotidewereemployedasprobesforSNPs.The sensingmechanismisbasedonthefluorescenceenhancementofsilvernanoclustersthroughan irreversibleclustertransferuponhybridization.Thekeyelementofourdesignismodulatingthe‘‘turn on’’ mechanismbyintroducingacompetitorthatcanbedisplacedinresponsetoatargetDNA.Ina controlledmodelsystem,thefluorescenceintensityofsilvernanoclustersisapproximately3-fold enhanceduponhybridizationwithaperfectlymatchedtargetDNA,andsingle-basemismatch detectionisachievedwithin5min,regardlessofthepositionofmismatches.Humanaldehyde dehydrogenase2(ALDH2),whichisresponsiblefortheoxidationofaldehydestocarboxylicacids,is then utilizedasatargetforSNP.Uponadditionofaperfectlymatchedsequence,dramaticallyenhanced fluorescenceintensityisobserved(ca.48-fold),andpromptSNPgenotypingisaccomplished. | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | Silver nanoclusters Single nucleotidepolymorphism(SNP) Fluorescent probesOligonucleotide Aldehyde dehydrogenase2(ALDH2) | - |
dc.title | An approach toward SNP detection by modulating the fluorescence of DNA-templated silver nanoclusters | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000316525300067 | - |
dc.identifier.scopusid | 2-s2.0-84873803454 | - |
dc.identifier.rimsid | 93 | ko |
dc.contributor.affiliatedAuthor | Juhee Park | - |
dc.contributor.affiliatedAuthor | Jihyun Lee | - |
dc.contributor.affiliatedAuthor | Won Jong Kim | - |
dc.identifier.doi | 10.1016/j.bios.2012.12.042 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.43, no.1, pp.419 - 424 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 43 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 419 | - |
dc.citation.endPage | 424 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |