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Chang, Young Tae
복잡계 자기조립 연구단
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ABCB1 Can Actively Pump-out the Background-Free Tame Fluorescent Probe CO-1 From Live Cells

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Title
ABCB1 Can Actively Pump-out the Background-Free Tame Fluorescent Probe CO-1 From Live Cells
Author(s)
Miasiro Ciaramicoli, Larissa; Seok Kim, Heon; Husen Alamudi, Samira; Young-Tae Chang
Publication Date
2022-06
Journal
Chemistry - An Asian Journal, v.17, no.12
Publisher
John Wiley and Sons Ltd
Abstract
© 2022 Wiley-VCH GmbH.Cell labelling using a small fluorescent probe is an important technique in biomedical sciences. We previously developed a biocompatible and membrane-permeable probe, CO-1, which has low nonspecific binding affinity towards nontarget molecules. Although this background-free tame probe has been utilized for labelling of various intracellular biomolecules in live cells, the probes’ backgroung-free staining mechanism was not fully understood. Here, we propose that Gating-Oriented Live-cell Distinction (GOLD) mechanism occurs when ABCB1 transporter removes unbound CO-1 molecules from mammalian cells and, in a minor role, DIRC2 pumps CO-1 out from lysosomes. We also showed that solute carrier transporters were not involved in carrying CO-1 inside of cells. The role of reporters in assisting the probes’ influx-efflux was analyzed by the combination of CRISPR library sceenings and inhibitors test. In summary, tame probe CO-1 cellular staining occurs in a dual mechanism where the probe moves freely through the cells membrane, but its washable property can be directly related to the action of ABCB1 transporter.
URI
https://pr.ibs.re.kr/handle/8788114/11920
DOI
10.1002/asia.202200229
ISSN
1861-4728
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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