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Chang, Young Tae
복잡계 자기조립 연구단
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ABCB1 Can Actively Pump-out the Background-Free Tame Fluorescent Probe CO-1 From Live Cells

DC Field Value Language
dc.contributor.authorMiasiro Ciaramicoli, Larissa-
dc.contributor.authorSeok Kim, Heon-
dc.contributor.authorHusen Alamudi, Samira-
dc.contributor.authorYoung-Tae Chang-
dc.date.accessioned2022-07-28T04:41:39Z-
dc.date.available2022-07-28T04:41:39Z-
dc.date.created2022-05-16-
dc.date.issued2022-06-
dc.identifier.issn1861-4728-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/11920-
dc.description.abstract© 2022 Wiley-VCH GmbH.Cell labelling using a small fluorescent probe is an important technique in biomedical sciences. We previously developed a biocompatible and membrane-permeable probe, CO-1, which has low nonspecific binding affinity towards nontarget molecules. Although this background-free tame probe has been utilized for labelling of various intracellular biomolecules in live cells, the probes’ backgroung-free staining mechanism was not fully understood. Here, we propose that Gating-Oriented Live-cell Distinction (GOLD) mechanism occurs when ABCB1 transporter removes unbound CO-1 molecules from mammalian cells and, in a minor role, DIRC2 pumps CO-1 out from lysosomes. We also showed that solute carrier transporters were not involved in carrying CO-1 inside of cells. The role of reporters in assisting the probes’ influx-efflux was analyzed by the combination of CRISPR library sceenings and inhibitors test. In summary, tame probe CO-1 cellular staining occurs in a dual mechanism where the probe moves freely through the cells membrane, but its washable property can be directly related to the action of ABCB1 transporter.-
dc.language영어-
dc.publisherJohn Wiley and Sons Ltd-
dc.titleABCB1 Can Actively Pump-out the Background-Free Tame Fluorescent Probe CO-1 From Live Cells-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000789594400001-
dc.identifier.scopusid2-s2.0-85129225413-
dc.identifier.rimsid78175-
dc.contributor.affiliatedAuthorYoung-Tae Chang-
dc.identifier.doi10.1002/asia.202200229-
dc.identifier.bibliographicCitationChemistry - An Asian Journal, v.17, no.12-
dc.relation.isPartOfChemistry - An Asian Journal-
dc.citation.titleChemistry - An Asian Journal-
dc.citation.volume17-
dc.citation.number12-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaChemistry-
dc.relation.journalWebOfScienceCategoryChemistry, Multidisciplinary-
dc.subject.keywordPlusLYSOSOMAL MEMBRANE-
dc.subject.keywordPlusTRANSPORTER-
dc.subject.keywordPlusPHARMACOGENETICS-
dc.subject.keywordPlusINHIBITOR-
dc.subject.keywordPlusPD173074-
dc.subject.keywordPlusGROWTH-
dc.subject.keywordPlusDIRC2-
dc.subject.keywordPlusGENE-
dc.subject.keywordAuthorABCB1-
dc.subject.keywordAuthorbackground-free cell staining-
dc.subject.keywordAuthorfluorescent probe-
dc.subject.keywordAuthorGating-Oriented Live-cell Distinction-
dc.subject.keywordAuthorTame probe-
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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