DNA-templated silver nanoclusters as label-free, sensitive detection probes for potassium ions and nitric oxide
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Title
- DNA-templated silver nanoclusters as label-free, sensitive detection probes for potassium ions and nitric oxide
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Author(s)
- Jihyun Lee; Junghong Park; Hong Hee Lee; Hugh I. Kim; Won Jong Kim
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Publication Date
- 2014-05
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Journal
- JOURNAL OF MATERIALS CHEMISTRY B, v.2, no.17, pp.2616 - 2621
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Publisher
- ROYAL SOC CHEMISTRY
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Abstract
- DNA-templated silver nanoclusters (DNA–AgNCs) have emerged as promising materials for sensing, biolabelling,
and bio-imaging due to their fluorogenic properties. Using the 12 mer DNA with a cytosine-rich
sequence, AgCNs have been formed successfully. Herein, we develop two different types of DNA–AgNC
systems for the detection of potassium ions (K+) and nitric oxide (NO) by utilizing the structural change of
DNA or DNA template transformation. In K+ detection, a thrombin binding aptamer (TBA) was utilized as
both the AgNC template and the K+ binding probe. The fluorescence emission of the DNA1–AgNC probe
was diminished proportionally to the amount of K+ detected, which transforms the AgNC-adapted
structure into G-quadruplex. On the other hand, NO detection has been accomplished by NO-induced
deamination of cytosine. The structural change of cytosine to deoxyuridine destabilizes the DNA2–AgNC
structure, resulting in quenching of fluorescence intensity. The characteristics of these two highly
quantitative detection probes were analysed by UV absorption, fluorescence emission, and transmission
electron microscopy (TEM). Each system provides a detection limit of 6.2 mM for K+ and 0.1 mM for NO.
We expect that these systems enable the opening of a new horizon for highly sensitive and selective
detection systems.
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URI
- https://pr.ibs.re.kr/handle/8788114/1184
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DOI
- 10.1039/c3tb21446j
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ISSN
- 2050-750X
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Appears in Collections:
- Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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