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Fluorescence polarization system for rapid COVID-19 diagnosis

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Title
Fluorescence polarization system for rapid COVID-19 diagnosis
Author(s)
Chang Yeol Lee; Ismail Degani; Jiyong Cheong; Jae-Hyun Lee; Hyun-Jung Choi; Jinwoo Cheon; Hakho Lee
Publication Date
2021-04
Journal
Biosensors and Bioelectronics, v.178
Publisher
Pergamon Press Ltd.
Abstract
Prompt diagnosis, patient isolation, and contact tracing are key measures to contain the coronavirus disease 2019 (COVID-19). Molecular tests are the current gold standard for COVID-19 detection, but are carried out at central laboratories, delaying treatment and control decisions. Here we describe a portable assay system for rapid, onsite COVID-19 diagnosis. Termed CODA (CRISPR Optical Detection of Anisotropy), the method combined isothermal nucleic acid amplification, activation of CRISPR/Cas12a, and signal generation in a single assay, eliminating extra manual steps. Importantly, signal detection was based on the ratiometric measurement of fluorescent anisotropy, which allowed CODA to achieve a high signal-to-noise ratio. For point-of-care operation, we built a compact, standalone CODA device integrating optoelectronics, an embedded heater, and a microcontroller for data processing. The developed system completed SARS-CoV-2 RNA detection within 20 min of sample loading; the limit of detection reached 3 copy/mu L. When applied to clinical samples (10 confirmed COVID-19 patients; 10 controls), the rapid CODA test accurately classified COVID-19 status, in concordance with gold-standard clinical diagnostics.
URI
https://pr.ibs.re.kr/handle/8788114/9819
DOI
10.1016/j.bios.2021.113049
ISSN
0956-5663
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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