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Fluorescence polarization system for rapid COVID-19 diagnosis

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dc.contributor.authorChang Yeol Lee-
dc.contributor.authorIsmail Degani-
dc.contributor.authorJiyong Cheong-
dc.contributor.authorJae-Hyun Lee-
dc.contributor.authorHyun-Jung Choi-
dc.contributor.authorJinwoo Cheon-
dc.contributor.authorHakho Lee-
dc.date.accessioned2021-07-02T02:50:04Z-
dc.date.accessioned2021-07-02T02:50:04Z-
dc.date.available2021-07-02T02:50:04Z-
dc.date.available2021-07-02T02:50:04Z-
dc.date.created2021-02-26-
dc.date.issued2021-04-
dc.identifier.issn0956-5663-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/9819-
dc.description.abstractPrompt diagnosis, patient isolation, and contact tracing are key measures to contain the coronavirus disease 2019 (COVID-19). Molecular tests are the current gold standard for COVID-19 detection, but are carried out at central laboratories, delaying treatment and control decisions. Here we describe a portable assay system for rapid, onsite COVID-19 diagnosis. Termed CODA (CRISPR Optical Detection of Anisotropy), the method combined isothermal nucleic acid amplification, activation of CRISPR/Cas12a, and signal generation in a single assay, eliminating extra manual steps. Importantly, signal detection was based on the ratiometric measurement of fluorescent anisotropy, which allowed CODA to achieve a high signal-to-noise ratio. For point-of-care operation, we built a compact, standalone CODA device integrating optoelectronics, an embedded heater, and a microcontroller for data processing. The developed system completed SARS-CoV-2 RNA detection within 20 min of sample loading; the limit of detection reached 3 copy/mu L. When applied to clinical samples (10 confirmed COVID-19 patients; 10 controls), the rapid CODA test accurately classified COVID-19 status, in concordance with gold-standard clinical diagnostics.-
dc.description.uri1-
dc.language영어-
dc.publisherPergamon Press Ltd.-
dc.titleFluorescence polarization system for rapid COVID-19 diagnosis-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000621206800002-
dc.identifier.scopusid2-s2.0-85100247110-
dc.identifier.rimsid74798-
dc.contributor.affiliatedAuthorChang Yeol Lee-
dc.contributor.affiliatedAuthorJiyong Cheong-
dc.contributor.affiliatedAuthorJae-Hyun Lee-
dc.contributor.affiliatedAuthorJinwoo Cheon-
dc.contributor.affiliatedAuthorHakho Lee-
dc.identifier.doi10.1016/j.bios.2021.113049-
dc.identifier.bibliographicCitationBiosensors and Bioelectronics, v.178-
dc.citation.titleBiosensors and Bioelectronics-
dc.citation.volume178-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalWebOfScienceCategoryBiophysics-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.relation.journalWebOfScienceCategoryChemistry, Analytical-
dc.relation.journalWebOfScienceCategoryElectrochemistry-
dc.relation.journalWebOfScienceCategoryNanoscience & Nanotechnology-
dc.subject.keywordAuthorBiosensor-
dc.subject.keywordAuthorCOVID-19-
dc.subject.keywordAuthorCRISPR/Cas-
dc.subject.keywordAuthorIsothermal amplification-
dc.subject.keywordAuthorFluorescence polarization-
dc.subject.keywordAuthorPoint-of-care-
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > 1. Journal Papers (저널논문)
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