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나노입자 연구단
나노입자 연구단
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Multi-modal transfection agent based on monodisperse magnetic nanoparticles for stem cell gene delivery and tracking

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Title
Multi-modal transfection agent based on monodisperse magnetic nanoparticles for stem cell gene delivery and tracking
Author(s)
Park W.; Yang H.N.; Daishun Ling; Yim H.; Kim K.S.; Taeg Hwan Hyeon; Na K.; Park K.-H.
Publication Date
2014-08
Journal
BIOMATERIALS, v.35, no.25, pp.7239 - 7247
Publisher
ELSEVIER SCI LTD
Abstract
Directing the controlled differentiation and tracking of stem cells is essential to achieve successful stem cell therapy. In this work, we describe a multi-modal (MR/optical) transfection agent (MTA) for efficient gene delivery and cell tracking of human mesenchymal stem cells (hMSCs). The MTA was synthesized through a facile two-step approach with 1) ligand exchange of a catechol-functionalized polypeptide (CFP) and 2) chemical immobilization of fluorescence labelled cationic polymer via aminolysis reaction. Cationic polymer-immobilized MTAs with size of ~40nm exhibit greatly enhanced colloidal stability in aqueous solution. In addition, the MTAs were capable of binding DNA molecules for transfection. The MTA/pDNA complex showed relatively good transfection efficiency in hMSCs (compared to the commercial transfection agent, Lipofectamine) and good biocompatibility. MTA-treated hMSCs were successfully visualized after transplantation via MR and optical imaging system over 14 days. These studies highlight the challenges associated with the potential advantages of designing multi-modal nanostructured materials as tools for genetic materials delivery and cell-tracking in stem cell therapy. © 2014 Elsevier Ltd.
URI
https://pr.ibs.re.kr/handle/8788114/929
ISSN
0142-9612
Appears in Collections:
Center for Nanoparticle Research(나노입자 연구단) > Journal Papers (저널논문)
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