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Green-, Red-, and Near-Infrared-Emitting Polymer Dot Probes for Simultaneous Multicolor Cell Imaging with a Single Excitation Wavelength

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Title
Green-, Red-, and Near-Infrared-Emitting Polymer Dot Probes for Simultaneous Multicolor Cell Imaging with a Single Excitation Wavelength
Author(s)
Jeong, Ji-Eun; Uddin, Mohammad Afsar; 분자 분광학 및 동력학 연구단; Hee-Chang Kim; Minsu Kang; Joung, Joonyoung Francis; Park, Sungnam; Sang-Hee Shim; Woo, Han Young
Publication Date
2020-08
Journal
CHEMISTRY OF MATERIALS, v.32, no.15, pp.6685 - 6696
Publisher
AMER CHEMICAL SOC
Abstract
We report newly synthesized fluorescence resonance energy transfer (FRET)-based green-, red-, and near-infrared (NIR)-emitting polymer dot (Pdot) probes. Fluorescent Pdots (similar to 60 nm) were prepared with a green-emissive conjugated polymer (PPDT-P, donor) alone or mixing the donor with a red- or NIR-emitting fluorophore (T-DCS or ITIC, acceptor), where an optically inert matrix polymer [poly(styrene-co-maleic anhydride)] was mixed together to minimize the aggregation-caused quenching by diluting the fluorophores and surface functionalization for further bioconjugation with antibodies for active targeting. Highly fluorescent green-emissive PPDT-P Pdots were prepared with a photoluminescence (PL) quantum efficiency of similar to 30% and the FRET-mediated red and NIR PL was intensified by 5.3-8.5 times (with high FRET ratios of similar to 9) via the efficient energy transfer (FRET efficiency of 80-98%) and antenna effect, compared with the signals obtained via direct excitation of the fluorophores in Pdots. All three types of Pdot/antibody conjugates were simultaneously immunostained to COS-7 cells (showing minimal cross-reactivity), reducing the tedious sequential immunostaining process to a single step. Finally, we obtained high-contrast three-color cell images with little spectral leakthrough by exciting all the probes simultaneously at a single wavelength at 405 nm without the need for a complicated or expensive multiple-excitation setup
URI
https://pr.ibs.re.kr/handle/8788114/8938
DOI
10.1021/acs.chemmater.0c02172
ISSN
0897-4756
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
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