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분자분광학및동력학연구단
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Green-, Red-, and Near-Infrared-Emitting Polymer Dot Probes for Simultaneous Multicolor Cell Imaging with a Single Excitation Wavelength

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dc.contributor.authorJeong, Ji-Eun-
dc.contributor.authorUddin, Mohammad Afsar-
dc.contributor.authorRyu, Hwa Sook-
dc.contributor.authorHee-Chang Kim-
dc.contributor.authorMinsu Kang-
dc.contributor.authorJoung, Joonyoung Francis-
dc.contributor.authorPark, Sungnam-
dc.contributor.authorSang-Hee Shim-
dc.contributor.authorWoo, Han Young-
dc.date.accessioned2020-12-31T01:50:02Z-
dc.date.accessioned2020-12-31T01:50:02Z-
dc.date.available2020-12-31T01:50:02Z-
dc.date.available2020-12-31T01:50:02Z-
dc.date.created2020-10-16-
dc.date.issued2020-08-
dc.identifier.issn0897-4756-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/8938-
dc.description.abstractWe report newly synthesized fluorescence resonance energy transfer (FRET)-based green-, red-, and near-infrared (NIR)-emitting polymer dot (Pdot) probes. Fluorescent Pdots (similar to 60 nm) were prepared with a green-emissive conjugated polymer (PPDT-P, donor) alone or mixing the donor with a red- or NIR-emitting fluorophore (T-DCS or ITIC, acceptor), where an optically inert matrix polymer [poly(styrene-co-maleic anhydride)] was mixed together to minimize the aggregation-caused quenching by diluting the fluorophores and surface functionalization for further bioconjugation with antibodies for active targeting. Highly fluorescent green-emissive PPDT-P Pdots were prepared with a photoluminescence (PL) quantum efficiency of similar to 30% and the FRET-mediated red and NIR PL was intensified by 5.3-8.5 times (with high FRET ratios of similar to 9) via the efficient energy transfer (FRET efficiency of 80-98%) and antenna effect, compared with the signals obtained via direct excitation of the fluorophores in Pdots. All three types of Pdot/antibody conjugates were simultaneously immunostained to COS-7 cells (showing minimal cross-reactivity), reducing the tedious sequential immunostaining process to a single step. Finally, we obtained high-contrast three-color cell images with little spectral leakthrough by exciting all the probes simultaneously at a single wavelength at 405 nm without the need for a complicated or expensive multiple-excitation setup-
dc.description.uri1-
dc.language영어-
dc.publisherAMER CHEMICAL SOC-
dc.titleGreen-, Red-, and Near-Infrared-Emitting Polymer Dot Probes for Simultaneous Multicolor Cell Imaging with a Single Excitation Wavelength-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000562136900042-
dc.identifier.scopusid2-s2.0-85090498772-
dc.identifier.rimsid73094-
dc.contributor.affiliatedAuthorHee-Chang Kim-
dc.contributor.affiliatedAuthorMinsu Kang-
dc.contributor.affiliatedAuthorSang-Hee Shim-
dc.identifier.doi10.1021/acs.chemmater.0c02172-
dc.identifier.bibliographicCitationCHEMISTRY OF MATERIALS, v.32, no.15, pp.6685 - 6696-
dc.citation.titleCHEMISTRY OF MATERIALS-
dc.citation.volume32-
dc.citation.number15-
dc.citation.startPage6685-
dc.citation.endPage6696-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.subject.keywordPlusCONJUGATED POLYELECTROLYTES-
dc.subject.keywordPlusENERGY-TRANSFER-
dc.subject.keywordPlusNANOPARTICLES-
dc.subject.keywordPlusEMISSION-
dc.subject.keywordPlusULTRABRIGHT-
dc.subject.keywordPlusDESIGN-
dc.subject.keywordPlusWATER-
dc.subject.keywordPlusBAND-
dc.subject.keywordAuthorSemiconducting Polymers-
dc.subject.keywordAuthorPhotoacoustics-
dc.subject.keywordAuthorBenzo-1,2,3-Thiadiazole-
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > 1. Journal Papers (저널논문)
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