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Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish

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Title
Near-Infrared Voltage Nanosensors Enable Real-Time Imaging of Neuronal Activities in Mice and Zebrafish
Author(s)
Jianan Liu; Zhang R.; Shang C.; Zhang Y.; Feng Y.; Pan L.; Xu B.; Taeghwan Hyeon; Bu W.; Shi J.; Du J.
Subject
LUMINESCENT GOLD NANOPARTICLES, ; UPCONVERTING NANOPARTICLES, ; ELECTRICAL-ACTIVITY, ; BRAIN-STIMULATION, ; MEMBRANE, ; NANOCRYSTALS, ; CALCIUM
Publication Date
2020-04
Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.142, no.17, pp.7858 - 7867
Publisher
AMER CHEMICAL SOC
Abstract
© 2020 American Chemical Society. Optical voltage sensors with the ability to monitor neuronal activities are invaluable tools for studying information processing of the brain. However, the current genetically encoded voltage indicators usually require high-power visible light for excitation and are limited to genetically addressable model animals. Here, we report a near-infrared (NIR)-excited nongenetic voltage nanosensor that achieves stable recording of neuronal membrane potential in intact animals. The nanosensor is composed of a Förster resonance energy transfer (FRET) pair, the outer membrane-anchored upconversion nanoparticle (UCNP), and the membrane-embedded dipicrylamine (DPA). The negative charge of DPA allows membrane potential fluctuation to affect the distance between the DPA and UCNP, therefore changing the FRET efficiency. Consequently, the emission intensity of the nanosensor can report the membrane potential. Using the nanosensor, we monitor not only electrically evoked changes in the membrane potential of cultured cells but also sensory responses of neurons in intact zebrafish and brain state-modulated subthreshold activities of cortical neurons in intact mice
URI
https://pr.ibs.re.kr/handle/8788114/8666
DOI
10.1021/jacs.0c01025
ISSN
0002-7863
Appears in Collections:
Center for Nanoparticle Research(나노입자 연구단) > 1. Journal Papers (저널논문)
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