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면역미생물공생연구단
면역 미생물 공생 연구단
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Oral immunization of haemaggulutinin H5 expressed in plant endoplasmic reticulum with adjuvant saponin protects mice against highly pathogenic avian influenza A virus infection

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Title
Oral immunization of haemaggulutinin H5 expressed in plant endoplasmic reticulum with adjuvant saponin protects mice against highly pathogenic avian influenza A virus infection
Author(s)
Goeun Lee; Yun Jeong Na; Bo-Gie Yang; Jun-Pyo Choi; Yong Bok Seo; Chun-Pyo Hong; Changho Yun; Dae Heon Kim; Eun Ju Sohn; Jeong Hee Kim; Young Chul Sung; Yoon-Keun Kim; Myoung Ho Jang; Inhwan Hwang
Publication Date
2015-01
Journal
PLANT BIOTECHNOLOGY JOURNAL, v.2014, no., pp.1 - 11
Publisher
WILEY-BLACKWELL
Abstract
Pandemics in poultry caused by the highly pathogenic avian influenza (HPAI) A virus occur too frequently globally, and there is growing concern about the HPAI A virus due to the possibility of a pandemic among humans. Thus, it is important to develop a vaccine against HPAI suitable for both humans and animals. Various approaches are underway to develop such vaccines. In particular, an edible vaccine would be a convenient way to vaccinate poultry because of the behaviour of the animals. However, an edible vaccine is still not available. In this study, we developed a strategy of effective vaccination of mice by the oral administration of transgenic Arabidopsis plants (HA-TG) expressing haemagglutinin (HA) in the endoplasmic reticulum (ER). Expression of HA in the ER resulted in its high-level accumulation, N-glycosylation, protection from proteolytic degradation and long-term stability. Oral administration of HA-TG with saponin elicited high levels of HA-specific systemic IgG and mucosal IgA responses in mice, which resulted in protection against a lethal influenza virus infection with attenuated inflammatory symptoms. Based on these results, we propose that oral administration of freeze-dried leaf powders from transgenic plants expressing HA in the ER together with saponin is an attractive strategy for vaccination against influenza A virus.
URI
https://pr.ibs.re.kr/handle/8788114/827
ISSN
1467-7644
Appears in Collections:
Academy of Immunology and Microbiology(면역 미생물 공생 연구단) > Journal Papers (저널논문)
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첨부12. Oral immunization of haemaggulutinin H5 expressed in plant endoplasmic reticulum.pdfDownload

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