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Chang, Young Tae
복잡계 자기조립 연구단
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Validation of CDr15 as a new dye for detecting neutrophil extracellular trap

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Title
Validation of CDr15 as a new dye for detecting neutrophil extracellular trap
Author(s)
Kim S.-J.; Kim J.; Kim B.; Lee W.-W.; Liu X.; Young-Tae Chang; Park J.-W.
Subject
CDr15, ; DNA dye, ; Fluorescence imaging, ; Neutrophil extracellular trap
Publication Date
2020-06
Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.527, no.3, pp.646 - 653
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Abstract
© 2020 Elsevier Inc. Neutrophil extracellular trap (NET) is one of the first-line defenses against microbes. Under certain circumstances, however, it also plays an aggravating factor in diverse inflammation-related diseases including cancers and vascular diseases. Our aim is to develop a new method to detect NET in cells and tissues using a DNA-specific fluorescence probe CDr15. CDr15 was characterized to be impermeable to the cell membranes and to emit a strong fluorescence in association with extracellular DNAs in NET. Due to these properties, CDr15 was successfully shown to quantify NETs in vitro and to be applicable for real-time monitoring NET formation in PMA-stimulated neutrophils. Even in formaldehyde-fixed tumor specimens, CDr15 could detect NETs spreading around cancer cells. Compared with DAPI and SYTOX DNA dyes, CDr15 showed a lower level of background fluorescence and a higher specificity in NET detection. Based on these results, we propose CDr15 as a novel marker of NET to be applicable in experimental and clinical studies
URI
https://pr.ibs.re.kr/handle/8788114/7844
DOI
10.1016/j.bbrc.2020.04.153
ISSN
0006-291X
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
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