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나노입자 연구단
나노입자 연구단
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A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform

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Title
A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform
Author(s)
Howon Lee; Hyoki Kim; Sungsik Kim; Taehoon Ryu; Hwangbeom Kim; Duhee Bang; Sunghoon Kwon
Publication Date
2015-02
Journal
NATURE COMMUNICATIONS, v.6, no., pp.6073 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Writing DNA plays a significant role in the fields of synthetic biology, functional genomics, and bioengineering. DNA clones on next generation sequencing (NGS) platforms have the potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. However, it is still very challenging to retrieve target clonal DNA from highdensity NGS platforms. Here, we propose an enabling technology called ‘Sniper Cloning’ that enables the precise mapping of target clone features on NGS platforms and non-contact rapid retrieval of targets for full utilization of DNA clones. By merging the three cutting-edge technologies of NGS, DNA microarray, and our pulse laser retrieval system, ‘Sniper Cloning’ is a week-long process by which 5,188 error-free synthetic DNAs can be produced in a single run of NGS with a single microarray DNA pool. We believe that this technology has potential as a universal tool for DNA writing in biological sciences.
URI
http://pr.ibs.re.kr/handle/8788114/741
DOI
10.1038/ncomms7073
ISSN
2041-1723
Appears in Collections:
Center for Nanoparticle Research(나노입자 연구단) > Journal Papers (저널논문)
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