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나노 구조 물리 연구단
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Identifying Fibrillization State of Aβ Protein via Near-Field THz Conductance Measurement

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Title
Identifying Fibrillization State of Aβ Protein via Near-Field THz Conductance Measurement
Author(s)
Chaejeong Heo; Taewoo Ha; Chunjae You; Thuy Huynh; Hosub Lim; Jiwon Kim; Mallikarjuna Reddy Kesama; Jinkee Lee; Teun-Teun Kim; Young Hee Lee
Publication Date
2020-06
Journal
ACS NANO, v.14, no.6, pp.6548 - 6558
Publisher
AMER CHEMICAL SOC
Abstract
Progressive Alzheimer's disease is correlated with the oligomerization and fibrillization of the amyloid beta (Aβ) protein. We identify the fibrillization stage of the Aβ protein through label-free near-field THz conductance measurements in a buffer solution. Frequency-dependent conductance was obtained by measuring the differential transmittance of the time-domain spectroscopy in the THz range with a molar concentration of monomer, oligomer, and fibrillar forms of the Aβ protein. Conductance at the lower frequency limit was observed to be high in monomers, reduced in oligomers, and dropped to an insulating state in fibrils and increased proportionally with the Aβ protein concentration. The monotonic decrease in the conductance at low frequency was dominated by a simple Drude component in the monomer with concentration and nonlinear conductance behaviors in the oligomer and fibril. By extracting the structural localization parameter, a dimensionless constant, with the modified Drude-Smith model, we defined a dementia quotient (DQ) value (0 < De < 1) as a discrete metric for a various Aβ proteins at a low concentration of 0.1 μmol/L; DQ = 1.0 ± 0.002 (fibril by full localization, mainly by Smith component), DQ = 0.64 ± 0.013 (oligomer by intermixed localization), and DQ = 0.0 ± 0.000 (monomer by Drude component). DQ values were discretely preserved independent of the molar concentration or buffer variation. This provides plenty of room for the label-free diagnosis of Alzheimer's disease using the near-field THz conductance measurement
URI
https://pr.ibs.re.kr/handle/8788114/7197
ISSN
1936-0851
Appears in Collections:
Center for Integrated Nanostructure Physics(나노구조물리 연구단) > Journal Papers (저널논문)
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