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복잡계 자기조립 연구단
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Supra-blot: An accurate and reliable assay for detecting target proteins with a synthetic host molecule-enzyme hybrid

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Title
Supra-blot: An accurate and reliable assay for detecting target proteins with a synthetic host molecule-enzyme hybrid
Author(s)
Gihyun Sung; Lee S.-Y.; Kang M.-G.; Kyung Lock Kim; An J.; Sim J.; Sungwan Kim; Kim S.; Ko J.; Rhee H.-W.; Kyeng Min Park; Kimoon Kim
Publication Date
2020-02
Journal
CHEMICAL COMMUNICATIONS, v.56, no.10, pp.1549 - 1552
Publisher
ROYAL SOC CHEMISTRY
Abstract
© 2020 The Royal Society of Chemistry.In accordance with the rapid increase in demand for selective and spatial chemical tagging, and accurate detection of proteins of interest, we develop a sensitive protein detection method, termed "Supra-blot" capitalizing on high-affinity host-guest interaction between cucurbit[7]uril (CB[7]) and adamantylammonium (AdA). The method can directly detect chemically tagged proteins without false-positive signals caused by endogenous biomolecules. Not only a single specific protein, but also spatially localized proteins in cells were labeled with AdA, and selectively detected by a host molecule-enzyme hybrid, CB[7]-conjugated horseradish peroxidase (CB[7]-HRP) generating amplified chemiluminescence signals. This study shows the great potential of Supra-blot for accurate and reliable detection of proteins of interest in cells
URI
https://pr.ibs.re.kr/handle/8788114/7000
ISSN
1359-7345
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
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