Steering Coacervation by a Pair of Broad-Spectrum Regulators

Cited 0 time in webofscience Cited 0 time in scopus
16 Viewed 6 Downloaded
Title
Steering Coacervation by a Pair of Broad-Spectrum Regulators
Author(s)
Shenyu Yang; Bo Li; Chunxian Wu; Weiwei Xu; Mei Tu; Yun Yan; Jianbin Huang; Markus Drechsler; Steve Granick; Lingxiang Jiang
Publication Date
2019-02
Journal
ACS NANO, v.13, no.2, pp.2420 - 2426
Publisher
AMER CHEMICAL SOC
Abstract
Coacervation is liquid-liquid phase separation ubiquitous in industrial applications and cellular biology. Inspired by cellular manipulation of coacervate droplets such as P granules, we report here a regulatory strategy to manipulate synthetic coacervation in a spatiotemporally controllable manner. Two oppositely charged small molecules are shown to phase separate into coacervate droplets in water as a result of electrostatic attraction, hydrophobic effect, and entropy. We identify a down regulator, β-cyclodextrin, to disrupt the hydrophobic effect, thus dissolving the droplets, and an up regulator, amylase, to decompose β-cyclodextrin, thus restoring the droplets. The regulation kinetics is followed in real time on a single-droplet level, revealing diffusion-limited dissolution and reaction-limited condensation, respectively, taking ∼4 s and 2-3 min. Versatility of this strategy to manipulate the coacervation is demonstrated in two aspects: spatially distributed coacervation in virtue of amylase-grafted hydrogel frameworks and coacervate transportation across membranes and hydrogel networks via a disassemble-to-pass strategy. The current regulatory pairs and strategies are anticipated to be general for a wide variety of synthetic self-assembly systems. Copyright © 2019 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/5702
ISSN
1936-0851
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
Files in This Item:
Steering Coacervation by a Pair of Broad-Spectrum Regulators.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse