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Anisha Shakya
첨단연성물질 연구단
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DNA Local-Flexibility-Dependent Assembly of Phase-Separated Liquid Droplets

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Title
DNA Local-Flexibility-Dependent Assembly of Phase-Separated Liquid Droplets
Author(s)
Anisha Shakya; John Thomas King
Publication Date
2018-11
Journal
BIOPHYSICAL JOURNAL, v.115, no.10, pp.1840 - 1847
Publisher
CELL PRESS
Abstract
Phase separation of intracellular components has been recently realized as a mechanism by which cells achieve membraneless organization. Here, we study the associative liquid-liquid phase separation (LLPS) of DNA upon complexation with cationic polypeptides. Comparing the phase behavior of different single-stranded DNA as well as double-stranded DNA (dsDNA) sequences that differ in persistence lengths, we find that DNA local flexibility, not simply charge density, determines the LLPS. Furthermore, in a nucleotide- and DNA-dependent manner, free nucleotide triphosphates promote LLPS of polypeptide-dsDNA complexes that are otherwise prone to precipitation. Under these conditions, dsDNA undergoes a secondary phase separation forming liquid-crystalline subcompartments inside the droplets. These results point toward a role of local DNA flexibility, encoded in the sequence, in the regulation and selectivity of multicomponent LLPS in membraneless intracellular organization. (c) 2018 Biophysical Society.
URI
https://pr.ibs.re.kr/handle/8788114/5073
ISSN
0006-3495
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
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