DNA Local-Flexibility-Dependent Assembly of Phase-Separated Liquid Droplets
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Anisha Shakya | - |
dc.contributor.author | John Thomas King | - |
dc.date.available | 2019-01-03T05:30:47Z | - |
dc.date.created | 2018-10-25 | - |
dc.date.issued | 2018-11 | - |
dc.identifier.issn | 0006-3495 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/5073 | - |
dc.description.abstract | Phase separation of intracellular components has been recently realized as a mechanism by which cells achieve membraneless organization. Here, we study the associative liquid-liquid phase separation (LLPS) of DNA upon complexation with cationic polypeptides. Comparing the phase behavior of different single-stranded DNA as well as double-stranded DNA (dsDNA) sequences that differ in persistence lengths, we find that DNA local flexibility, not simply charge density, determines the LLPS. Furthermore, in a nucleotide- and DNA-dependent manner, free nucleotide triphosphates promote LLPS of polypeptide-dsDNA complexes that are otherwise prone to precipitation. Under these conditions, dsDNA undergoes a secondary phase separation forming liquid-crystalline subcompartments inside the droplets. These results point toward a role of local DNA flexibility, encoded in the sequence, in the regulation and selectivity of multicomponent LLPS in membraneless intracellular organization. (c) 2018 Biophysical Society. | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | CELL PRESS | - |
dc.title | DNA Local-Flexibility-Dependent Assembly of Phase-Separated Liquid Droplets | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000450765100002 | - |
dc.identifier.scopusid | 2-s2.0-85054683342 | - |
dc.identifier.rimsid | 65873 | - |
dc.contributor.affiliatedAuthor | Anisha Shakya | - |
dc.contributor.affiliatedAuthor | John Thomas King | - |
dc.identifier.doi | 10.1016/j.bpj.2018.09.022 | - |
dc.identifier.bibliographicCitation | BIOPHYSICAL JOURNAL, v.115, no.10, pp.1840 - 1847 | - |
dc.citation.title | BIOPHYSICAL JOURNAL | - |
dc.citation.volume | 115 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1840 | - |
dc.citation.endPage | 1847 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | SINGLE-STRANDED-DNA | - |
dc.subject.keywordPlus | COMPLEX COACERVATION | - |
dc.subject.keywordPlus | ELECTROSTATICS | - |