A magnetic resonance tuning sensor for the MRI detection of biological targets

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Title
A magnetic resonance tuning sensor for the MRI detection of biological targets
Author(s)
Tae-Hyun Shin; Sunghwi Kang; Sohyeon Park; Jin-sil Choi; Pan Ki Kim; Jinwoo Cheon
Publication Date
2018-11
Journal
NATURE PROTOCOLS, v.13, no.11, pp.2664 - 2684
Publisher
NATURE PUBLISHING GROUP
Abstract
Sensors that detect specific molecules of interest in a living organism can be useful tools for studying biological functions and diseases. Here, we provide a protocol for the construction of nanosensors that can non-invasively detect biologically important targets with magnetic resonance imaging (MRI). The key operating principle of these sensors is magnetic resonance tuning (MRET), a distance-dependent phenomenon occurring between a superparamagnetic quencher and a paramagnetic enhancer. The change in distance between the two magnetic components modulates the longitudinal (T-1) relaxivity of the enhancer. In this MRET sensor, distance variation is achieved by interactive linkers that undergo binding, cleavage, or folding/unfolding upon their interaction with target molecules. By the modular incorporation of suitable linkers, the MRET sensor can be applied to a wide range of targets. We showcase three examples of MRET sensors for enzymes, nucleic acid sequences, and pH. This protocol comprises three stages: (i) chemical synthesis and surface modification of the quencher, (ii) conjugation with interactive linkers and enhancers, and (iii) MRI sensing of biological targets. The entire procedure takes up to 3 d
URI
https://pr.ibs.re.kr/handle/8788114/5043
ISSN
1754-2189
Appears in Collections:
Center for Nanomedicine (나노의학 연구단) > Journal Papers
Files in This Item:
201810_Nat. Protoc._A magnetic resonance tuning sensor for the MRI detection of biological targets.pdfDownload

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