A magnetic resonance tuning sensor for the MRI detection of biological targets

DC Field Value Language
dc.contributor.authorTae-Hyun Shin-
dc.contributor.authorSunghwi Kang-
dc.contributor.authorSohyeon Park-
dc.contributor.authorJin-sil Choi-
dc.contributor.authorPan Ki Kim-
dc.contributor.authorJinwoo Cheon-
dc.date.available2019-01-03T05:30:15Z-
dc.date.created2018-11-20-
dc.date.issued2018-11-
dc.identifier.issn1754-2189-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/5043-
dc.description.abstractSensors that detect specific molecules of interest in a living organism can be useful tools for studying biological functions and diseases. Here, we provide a protocol for the construction of nanosensors that can non-invasively detect biologically important targets with magnetic resonance imaging (MRI). The key operating principle of these sensors is magnetic resonance tuning (MRET), a distance-dependent phenomenon occurring between a superparamagnetic quencher and a paramagnetic enhancer. The change in distance between the two magnetic components modulates the longitudinal (T-1) relaxivity of the enhancer. In this MRET sensor, distance variation is achieved by interactive linkers that undergo binding, cleavage, or folding/unfolding upon their interaction with target molecules. By the modular incorporation of suitable linkers, the MRET sensor can be applied to a wide range of targets. We showcase three examples of MRET sensors for enzymes, nucleic acid sequences, and pH. This protocol comprises three stages: (i) chemical synthesis and surface modification of the quencher, (ii) conjugation with interactive linkers and enhancers, and (iii) MRI sensing of biological targets. The entire procedure takes up to 3 d-
dc.languageENG-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleA magnetic resonance tuning sensor for the MRI detection of biological targets-
dc.typeArticle-
dc.type.rimsA-
dc.identifier.wosid000448980400011-
dc.identifier.scopusids2.0-85055468430-
dc.contributor.affiliatedAuthorTae-Hyun Shin-
dc.contributor.affiliatedAuthorJinwoo Cheon-
dc.identifier.bibliographicCitationNATURE PROTOCOLS, v.13, no.11, pp.2664 - 2684-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
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Center for Nanomedicine (나노의학 연구단) > Journal Papers
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