A palette of background-free tame fluorescent probes for intracellular multi-color labelling in live cells

Cited 0 time in webofscience Cited 0 time in scopus
88 Viewed 58 Downloaded
Title
A palette of background-free tame fluorescent probes for intracellular multi-color labelling in live cells
Author(s)
Alamudi S.H.; Su D.; Lee K.J.; Lee J.Y.; Belmonte-Vázquez J.L.; Park H.-S.; Peña-Cabrera E.; Chang Y.-T.
Publication Date
2018-02
Journal
CHEMICAL SCIENCE, v.9, no.8, pp.2376 - 2383
Publisher
ROYAL SOC CHEMISTRY
Abstract
A multi-color labelling technique for visualizing multiple intracellular apparatuses in their native environment using small fluorescent probes remains challenging. This approach requires both orthogonal and biocompatible coupling reactions in heterogeneous biological systems with minimum fluorescence background noise. Here, we present a palette of BODIPY probes containing azide and cyclooctyne moieties for copper-free click chemistry in living cells. The probes, referred to as 'tame probes', are highly permeable and specific in nature, leaving no background noise in cells. Such probes, which are rationally designed through optimized lipophilicity, water solubility and charged van der Waals surface area, allow us to demonstrate rapid and efficient concurrent multi-labelling of intracellular target components. We show that these probes are capable of not only labelling organelles and engineered proteins, but also showing the intracellular glycoconjugates' dynamics, through the use of metabolic oligosaccharide engineering technology in various cell types. The results demonstrated in this study thus provide flexibility for multi-spectral labelling strategies in native systems in a high spatiotemporal manner. © 2018 The Royal Society of Chemistry
URI
https://pr.ibs.re.kr/handle/8788114/5009
ISSN
2041-6520
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
Files in This Item:
07.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse