A fluorescent chemical probe CDy9 selectively stains and enables the isolation of live naïve mouse embryonic stem cells

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Title
A fluorescent chemical probe CDy9 selectively stains and enables the isolation of live naïve mouse embryonic stem cells
Author(s)
Seung-Ju Cho; Keun-Tae Kim; Jong-Soo Kim; Ok-Seon Kwon; Young-hyun Go; Nam-young Kang; Haejeong Heo; Mi-Rang Kim; Dong Wook Han; Sung-Hwan Moon; Chang Y.-T.; Hyuk-Jin Cha
Publication Date
2018-10
Journal
BIOMATERIALS, v.180, no., pp.12 - 23
Publisher
ELSEVIER SCI LTD
Abstract
Human and mouse embryonic stem cells (ESCs) differ in terms of their pluripotency status, i.e., naïve vs. primed. This affects various biological properties and leads to several technical hurdles for future clinical applications, such as difficulties in chimera formation, single-cell passaging, and gene editing. In terms of generating functional human tissues and organs via mammalian interspecies chimerism, a fluorescent chemical probe that specifically labels naïve ESCs would help to isolate these cells and monitor their conversion. This study demonstrates that the fluorescent chemical probe compound of designation yellow 9 (CDy9) selectively stains naïve, but not primed, mouse ESCs (mESCs). CDy9 entered cells via Slc13a5, a highly expressed membrane transporter in naïve mESCs. Fluorescence-based cell sorting based on CDy9 staining successfully separated naïve mESCs from primed mESCs. Mice generated using CDy9+ cells isolated during the conversion of mouse epiblast stem cells into naïve mESCs exhibited coat color chimerism. Furthermore, CDy9 specifically stained cells in the inner cell mass of mouse embryos. These findings suggest that CDy9 is a useful tool to isolate functional naïve mESCs. © 2018 Published by Elsevier Ltd.
URI
https://pr.ibs.re.kr/handle/8788114/4995
ISSN
0142-9612
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
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