Supramolecular latching system based on ultrastable synthetic binding pairs as versatile tools for protein imaging

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Title
Supramolecular latching system based on ultrastable synthetic binding pairs as versatile tools for protein imaging
Author(s)
Kyung Lock Kim; Gihyun Sung; Jaehwan Sim; James Murray; Meng Li; Ara Lee; Annadka Shrinidhi; Kyeng Min Park; Kimoon Kim
Publication Date
2018-04
Journal
NATURE COMMUNICATIONS, v.9, no.1, pp.1712 -
Publisher
NATURE PUBLISHING GROUP
Abstract
Here we report ultrastable synthetic binding pairs between cucurbit[7]uril (CB[7]) and adamantyl- (AdA) or ferrocenyl-ammonium (FcA) as a supramolecular latching system for protein imaging, overcoming the limitations of protein-based binding pairs. Cyanine 3-conjugated CB[7] (Cy3-CB[7]) can visualize AdA- or FcA-labeled proteins to provide clear fluorescence images for accurate and precise analysis of proteins. Furthermore, controllability of the system is demonstrated by treating with a stronger competitor guest. At low temperature, this allows us to selectively detach Cy3-CB[7] from guest-labeled proteins on the cell surface, while leaving Cy3-CB[7] latched to the cytosolic proteins for spatially conditional visualization of target proteins. This work represents a non-protein-based bioimaging tool which has inherent advantages over the widely used protein-based techniques, thereby demonstrating the great potential of this synthetic system. © 2018 The Author(s)
URI
https://pr.ibs.re.kr/handle/8788114/4993
ISSN
2041-1723
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
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