BROWSE

Related Scientist

kim,kyunglock's photo.

kim,kyunglock
복잡계자기조립연구단
more info

ITEM VIEW & DOWNLOAD

Supramolecular latching system based on ultrastable synthetic binding pairs as versatile tools for protein imaging

DC Field Value Language
dc.contributor.authorKyung Lock Kim-
dc.contributor.authorGihyun Sung-
dc.contributor.authorJaehwan Sim-
dc.contributor.authorJames Murray-
dc.contributor.authorMeng Li-
dc.contributor.authorAra Lee-
dc.contributor.authorAnnadka Shrinidhi-
dc.contributor.authorKyeng Min Park-
dc.contributor.authorKimoon Kim-
dc.date.available2018-12-13T10:46:29Z-
dc.date.created2018-06-11-
dc.date.issued2018-04-
dc.identifier.issn2041-1723-
dc.identifier.urihttps://pr.ibs.re.kr/handle/8788114/4993-
dc.description.abstractHere we report ultrastable synthetic binding pairs between cucurbit[7]uril (CB[7]) and adamantyl- (AdA) or ferrocenyl-ammonium (FcA) as a supramolecular latching system for protein imaging, overcoming the limitations of protein-based binding pairs. Cyanine 3-conjugated CB[7] (Cy3-CB[7]) can visualize AdA- or FcA-labeled proteins to provide clear fluorescence images for accurate and precise analysis of proteins. Furthermore, controllability of the system is demonstrated by treating with a stronger competitor guest. At low temperature, this allows us to selectively detach Cy3-CB[7] from guest-labeled proteins on the cell surface, while leaving Cy3-CB[7] latched to the cytosolic proteins for spatially conditional visualization of target proteins. This work represents a non-protein-based bioimaging tool which has inherent advantages over the widely used protein-based techniques, thereby demonstrating the great potential of this synthetic system. © 2018 The Author(s)-
dc.description.uri1-
dc.language영어-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleSupramolecular latching system based on ultrastable synthetic binding pairs as versatile tools for protein imaging-
dc.typeArticle-
dc.type.rimsART-
dc.identifier.wosid000431009200004-
dc.identifier.scopusid2-s2.0-85046131039-
dc.identifier.rimsid63580-
dc.contributor.affiliatedAuthorKyung Lock Kim-
dc.contributor.affiliatedAuthorJames Murray-
dc.contributor.affiliatedAuthorMeng Li-
dc.contributor.affiliatedAuthorAnnadka Shrinidhi-
dc.contributor.affiliatedAuthorKyeng Min Park-
dc.contributor.affiliatedAuthorKimoon Kim-
dc.identifier.doi10.1038/s41467-018-04161-4-
dc.identifier.bibliographicCitationNATURE COMMUNICATIONS, v.9, no.1, pp.1712-
dc.citation.titleNATURE COMMUNICATIONS-
dc.citation.volume9-
dc.citation.number1-
dc.citation.startPage1712-
dc.description.journalClass1-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > 1. Journal Papers (저널논문)
Files in This Item:
11.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse