Dextran adsorption onto red blood cells revisited: Single cell quantification by laser tweezers combined with microfluidics

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Title
Dextran adsorption onto red blood cells revisited: Single cell quantification by laser tweezers combined with microfluidics
Author(s)
KISUNG LEE; EVGENY SHIRSHIN; NATALIYA ROVNYAGINA; FRANCOIS YAYA; ZAKARIA BOUJJA; ALEXANDER PRIEZZHEV; CHRISTIAN WAGNER
Publication Date
2018-06
Journal
Biomedical Optics Express, v.9, no.6, pp.2755 - 2764
Publisher
OPTICAL SOC AMER
Abstract
The aggregation of red blood cells (RBC) is of importance for hemorheology, while its mechanism remains debatable. The key question is the role of the adsorption of macromolecules on RBC membranes, which may act as “bridges” between cells. It is especially important that dextran is considered to induce “bridge”-less aggregation due to the depletion forces. We revisit the dextran-RBC interaction on the single cell level using the laser tweezers combined with microfluidic technology and fluorescence microscopy. An immediate sorption of ~104 molecules of 70 kDa dextran per cell was observed. During the incubation of RBC with dextran, a gradual tenfold increase of adsorption was found, accompanied by a moderate change in the RBC deformability. The obtained data demonstrate that dextran sorption and incubation-induced changes of the membrane properties must be considered when studying RBC aggregation in vitro. © 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
URI
https://pr.ibs.re.kr/handle/8788114/4981
ISSN
2156-7085
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
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