BROWSE

Related Scientist

Researcher

복잡계 자기조립 연구단
복잡계 자기조립 연구단
more info

Di-Sulfide Linked Polyethylenimine Coated Gold Nanoparticles as a Non-Viral Gene Delivery Agent in NIH-3T3 Mouse Embryonic Fibroblast

Cited 3 time in webofscience Cited 0 time in scopus
54 Viewed 2 Downloaded
Title
Di-Sulfide Linked Polyethylenimine Coated Gold Nanoparticles as a Non-Viral Gene Delivery Agent in NIH-3T3 Mouse Embryonic Fibroblast
Author(s)
Uthaman, S; Moon, MJ; Duhwan Lee; Won Jong Kim; Park, IK
Publication Date
2015-10
Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.15, no.10, pp.7895 - 7899
Publisher
AMER SCIENTIFIC PUBLISHERS
Abstract
Di-sulfide linked polyethylenimine coated gold nanoparticles (ssPEI-GNPs) of 20 nm size was prepared in order to deliver the genes to target site. DLS and TEM analysis demonstrated that the GNPs have average size of 13 nm in diameter. Upon coating the GNPs with ssPEI in the weight ratio of 1:3, the average hydrodynamic diameter of the ssPEI-GNPs was found to 19 +/- 1.14 nm and a zeta potential value 41 +/- 1.23 mV was observed. TEM analysis of ssPEI-GNPs demonstrated that the nanoparticles have spherical morphology. Thermogravemetric analysis of the prepared ssPEI-GNPs showed that the estimated composition of the ssPEI coated over the GNPs was approximately 5% (w/w). Gene expression capabilities of the nanoparticles were confirmed by fluorescent microscopy and luciferase assay, which demonstrated the transgene delivery capability of the ssPEI-GNPs. These results demonstrate that ssPEI-GNPs could be used as gene delivery agent © 2015 American Scientific Publishers
URI
https://pr.ibs.re.kr/handle/8788114/4852
ISSN
1533-4880
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
Files in This Item:
uthaman2015.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse