Unveiling the pathway to Z-DNA in the protein-induced B–Z transition

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Title
Unveiling the pathway to Z-DNA in the protein-induced B–Z transition
Author(s)
Sook Ho Kim; So-Hee Lim; Ae-Ree Lee; Do Hoon Kwon; Hyun Kyu Song; Joon-Hwa Lee; Minheang Cho; Albert Johner; Nam-Kyung Lee; Seok-Cheol Hong
Publication Date
2018-05
Journal
NUCLEIC ACIDS RESEARCH, v.46, no.8, pp.4129 - 4137
Publisher
OXFORD UNIV PRESS
Abstract
Left-handed Z-DNA is an extraordinary conformation of DNA, which can form by special sequences under specific biological, chemical or physical conditions. Human ADAR1, prototypic Z-DNA binding protein (ZBP), binds to Z-DNA with high affinity. Utilizing single-molecule FRET assays for Z-DNA forming sequences embedded in a long inactive DNA, we measure thermodynamic populations of ADAR1-bound DNA conformations in both GC and TG repeat sequences. Based on a statistical physics model, we determined quantitatively the affinities of ADAR1 to both Z-form and B-form of these sequences. We also reported what pathways it takes to induce the B–Z transition in those sequences. Due to the high junction energy, an intermediate B* state has to accumulate prior to the B–Z transition. Our study showing the stable B* state supports the active picture for the protein-induced B–Z transition that occurs under a physiological setting. (c)The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.
URI
https://pr.ibs.re.kr/handle/8788114/4534
ISSN
0305-1048
Appears in Collections:
Center for Molecular Spectroscopy and Dynamics(분자 분광학 및 동력학 연구단) > Journal Papers (저널논문)
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