Unveiling the pathway to Z-DNA in the protein-induced B–Z transition
DC Field | Value | Language |
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dc.contributor.author | Sook Ho Kim | - |
dc.contributor.author | So-Hee Lim | - |
dc.contributor.author | Ae-Ree Lee | - |
dc.contributor.author | Do Hoon Kwon | - |
dc.contributor.author | Hyun Kyu Song | - |
dc.contributor.author | Joon-Hwa Lee | - |
dc.contributor.author | Minheang Cho | - |
dc.contributor.author | Albert Johner | - |
dc.contributor.author | Nam-Kyung Lee | - |
dc.contributor.author | Seok-Cheol Hong | - |
dc.date.available | 2018-07-18T02:03:19Z | - |
dc.date.created | 2018-06-15 | - |
dc.date.issued | 2018-05 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://pr.ibs.re.kr/handle/8788114/4534 | - |
dc.description.abstract | Left-handed Z-DNA is an extraordinary conformation of DNA, which can form by special sequences under specific biological, chemical or physical conditions. Human ADAR1, prototypic Z-DNA binding protein (ZBP), binds to Z-DNA with high affinity. Utilizing single-molecule FRET assays for Z-DNA forming sequences embedded in a long inactive DNA, we measure thermodynamic populations of ADAR1-bound DNA conformations in both GC and TG repeat sequences. Based on a statistical physics model, we determined quantitatively the affinities of ADAR1 to both Z-form and B-form of these sequences. We also reported what pathways it takes to induce the B–Z transition in those sequences. Due to the high junction energy, an intermediate B* state has to accumulate prior to the B–Z transition. Our study showing the stable B* state supports the active picture for the protein-induced B–Z transition that occurs under a physiological setting. (c)The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. | - |
dc.description.uri | 1 | - |
dc.language | 영어 | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | Nucleic Acid Enzymes | - |
dc.title | Unveiling the pathway to Z-DNA in the protein-induced B–Z transition | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.identifier.wosid | 000431895800034 | - |
dc.identifier.scopusid | 2-s2.0-85051692148 | - |
dc.identifier.rimsid | 63755 | ko |
dc.contributor.affiliatedAuthor | Sook Ho Kim | - |
dc.contributor.affiliatedAuthor | So-Hee Lim | - |
dc.contributor.affiliatedAuthor | Do Hoon Kwon | - |
dc.contributor.affiliatedAuthor | Hyun Kyu Song | - |
dc.contributor.affiliatedAuthor | Minheang Cho | - |
dc.contributor.affiliatedAuthor | Seok-Cheol Hong | - |
dc.identifier.doi | 10.1093/nar/gky200 | - |
dc.identifier.bibliographicCitation | NUCLEIC ACIDS RESEARCH, v.46, no.8, pp.4129 - 4137 | - |
dc.citation.title | NUCLEIC ACIDS RESEARCH | - |
dc.citation.volume | 46 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 4129 | - |
dc.citation.endPage | 4137 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |