BROWSE

Related Scientist

Researcher

나노입자 연구단
나노입자 연구단
more info

Reversible Cell Layering for Heterogeneous Cell Assembly Mediated by Ionic Cross-Linking of Chitosan and a Functionalized Cell Surface Membrane

Cited 0 time in webofscience Cited 0 time in scopus
97 Viewed 4 Downloaded
Title
Reversible Cell Layering for Heterogeneous Cell Assembly Mediated by Ionic Cross-Linking of Chitosan and a Functionalized Cell Surface Membrane
Author(s)
Seungmi Ryu; Hyunbum Kim; Seokyung Kang; Kwangsoo Shin; Seon-Yeop Jung; Jiwoong Heo; Jin Han; Jeong-Kee Yoon; Ju-Ro Lee; Jinkee Hong; Kyung Hyun Ahn; Taeghwan Hyeon; Nathaniel Suk-Yeon Hwang; Byung-Soo Kim
Publication Date
2017-06
Journal
CHEMISTRY OF MATERIALS, v.29, no.12, pp.5294 - 5305
Publisher
AMER CHEMICAL SOC
Abstract
Current heterogeneous cell assembly techniques in coculture systems rely on irreversible cell layering or a cell separation membrane. However, the techniques possess major drawbacks of inefficiency in direct interactions of the assembled cell layers and cell separation following coculture, which hamper characterization and therapeutic applications of the cells following coculture. Here, we develop a reversible cell layering platform for assembly of heterogeneous cells that allows both active direct cell cell interactions and facile cell separation. Anionic maleimide-chondroitin-sulfate is grafted onto the surface membrane of myogenic C2C12 cells and human mesenchymal stem cells (hMSCs) to modify the surface charge of the cells without cytotoxicity. A highly porous chitosan thin film is formed in situ interspacing between the heterogeneous cell layers via ionic cross-linking of cationic chitosan and anionic functionalized cells, forming compactly assembled double-layered cell constructs. The chitosan film enables layering of the cells, which allows active direct interactions between the cell layers, and facile delayering of the cells through simple treatment with mild shear stress. The developed platform promotes the myogenic commitment of hMSCs via direct contact with C2C12 cells, mimicking the interactions that trigger stem cell differentiation in vivo. Delivery of the myogenic committed cells to muscle injured animal models shows evident muscle regeneration. © 2017 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/4247
ISSN
0897-4756
Appears in Collections:
Center for Nanoparticle Research(나노입자 연구단) > Journal Papers (저널논문)
Files in This Item:
02.reversible cell layering.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse