BROWSE

Related Scientist

Researcher

현택환
나노입자 연구단
more info

In Situ Quantification and Profiling of Phosphatidylcholine in Mouse Brain Tissue by Matrix-assisted Laser Desorption Ionization with a Liquid Matrix

Cited 0 time in webofscience Cited 0 time in scopus
92 Viewed 3 Downloaded
Title
In Situ Quantification and Profiling of Phosphatidylcholine in Mouse Brain Tissue by Matrix-assisted Laser Desorption Ionization with a Liquid Matrix
Author(s)
Kyung Man Park; Taeghwan Hyeon; Myung Soo Kim; Jeong Hee Moon
Publication Date
2017-06
Journal
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.38, no.6, pp.636 - 641
Publisher
KOREAN CHEMICAL SOC
Abstract
A method based on the analyte-to-matrix ion abundance ratio in the matrix-assisted laser desorption ionization (MALDI) spectrum was used for the quantification of phosphatidylcholine (PC) in mouse brain tissue. The fact that the tissue was heavily contaminated and hence that the matrix suppression was unacceptably high forbade the in situ quantification of PC. We eliminated most of the polar contaminants, and some nonpolar ones as well, by washing the tissue with water. In the process, we observed the presence of two different populations of PC, one washable in water and the other unwashable. Finally, we present a quantitative profile of the unwashable PC in a mouse brain tissue evaluated from its MALDI spectral data. © 2017 Korean Chemical Society, Seoul & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
URI
https://pr.ibs.re.kr/handle/8788114/4212
ISSN
0253-2964
Appears in Collections:
Center for Nanoparticle Research(나노입자 연구단) > Journal Papers (저널논문)
Files in This Item:
국내-01.in situ quantification.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse