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Stimuli-Regulated Enzymatically Degradable Smart Graphene-Oxide-Polymer Nanocarrier Facilitating Photothermal Gene Delivery

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Title
Stimuli-Regulated Enzymatically Degradable Smart Graphene-Oxide-Polymer Nanocarrier Facilitating Photothermal Gene Delivery
Author(s)
Hyunwoo Kim; Jinhwan Kim; Minkyung Lee; Hee Cheul Choi; Won Jong Kim
Publication Date
2016-08
Journal
ADVANCED HEALTHCARE MATERIALS, v.5, no.15, pp.1918 - 1930
Publisher
WILEY-BLACKWELL
Abstract
Graphene oxide (GO) has attracted huge interest in the area of biomedical application due to its unique physicochemical properties, but the issue of long-term toxicity in the body remains unclear. Here, the rationally designed GO nanocarriers (ssPEG-PEI-GO) modified with polyethylene glycol (PEG) and branched polyethylenimine (BPEI) via disulfide linkage are described to control the biological activity of GO as a delivery carrier and its degradation in biological systems. The ssPEG-PEI-GO efficiently interacts with plasmid DNA (pDNA) to form a stable nanocomplex by electrostatic interaction. After cellular uptake, ssPEG-PEI-GO/pDNA complex can easily escape from endosomes by photothermal conversion of GO upon near-infrared irradiation and subsequent photothermally induced endosome disruption. After endosomal escape, reducing intracellular environment enables polymer dissociation and rapid gene release and therefore shows enhanced gene transfection efficiency with low toxicity in comparison with non-reducible amide-functionalized GO nanocarriers (amPEG-PEI-GO) and control BPEIs. Besides, dePEGylated GO nanocarrier, owing to its disulfide bond, exhibits higher entrapment by macrophages compared with amide-functionalized one and subsequently degrades in macrophage. The degradation process can be monitored by photoluminescence emitted from degraded GO. These results suggest new directions in the design of biodegradable and multifunctional GO-based nanocarrier for biomedical application. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
URI
https://pr.ibs.re.kr/handle/8788114/3286
ISSN
2192-2640
Appears in Collections:
Center for Artificial Low Dimensional Electronic Systems(원자제어 저차원 전자계 연구단) > Journal Papers (저널논문)
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
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