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첨단연성물질 연구단
첨단연성물질 연구단
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Label-free, multi-scale imaging of ex-vivo mouse brain using spatial light interference microscopy

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Title
Label-free, multi-scale imaging of ex-vivo mouse brain using spatial light interference microscopy
Author(s)
Eunjung Min; Mikhail E. Kandel; CheMyong J Ko; Gabriel Popescu; Woonggyu Jung; Catherine Best-Popescu
Publication Date
2016-12
Journal
SCIENTIFIC REPORTS, v.6, no., pp.39667 -
Abstract
Brain connectivity spans over broad spatial scales, from nanometers to centimeters. In order to understand the brain at multi-scale, the neural network in wide-field has been visualized in detail by taking advantage of light microscopy. However, the process of staining or addition of fluorescent tags is commonly required, and the image contrast is insufficient for delineation of cytoarchitecture. To overcome this barrier, we use spatial light interference microscopy to investigate brain structure with high-resolution, sub-nanometer pathlength sensitivity without the use of exogenous contrast agents. Combining wide-field imaging and a mosaic algorithm developed in-house, we show the detailed architecture of cells and myelin, within coronal olfactory bulb and cortical sections, and from sagittal sections of the hippocampus and cerebellum. Our technique is well suited to identify laminar characteristics of fiber tract orientation within white matter, e.g. the corpus callosum. To further improve the macro-scale contrast of anatomical structures, and to better differentiate axons and dendrites from cell bodies, we mapped the tissue in terms of its scattering property. Based on our results, we anticipate that spatial light interference microscopy can potentially provide multiscale and multicontrast perspectives of gross and microscopic brain anatomy. © The Author(s) 2016
URI
http://pr.ibs.re.kr/handle/8788114/3219
DOI
10.1038/srep39667
ISSN
2045-2322
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
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