BROWSE

Related Scientist

Researcher

첨단연성물질 연구단
첨단연성물질 연구단
more info

One-photon and two-photon stimulation of neurons in a microfluidic culture system

Cited 3 time in webofscience Cited 0 time in scopus
277 Viewed 116 Downloaded
Title
One-photon and two-photon stimulation of neurons in a microfluidic culture system
Author(s)
Jang, JM; Lee, J; Kim, H; Jeon, NL; Woonggyu Jung
Publication Date
2016-01
Journal
LAB ON A CHIP, v.16, no.9, pp.1684 - 1690
Publisher
ROYAL SOC CHEMISTRY
Abstract
In this study, we demonstrate a novel platform for optical stimulation of neural circuits combined with a microfluidic culture method and microelectrode array measurements. Neuron-on-a-chip was designed and fabricated to isolate axons without a soma or dendrite. Thus, it is readily able to manipulate the neuronal alignment and to investigate the neuronal activity at the locations we want to observe. We adapted the optical stimulation technique to the arranged neurons to generate the neuronal signals in a non-invasive fashion. A blue light-emitting diode and a femtosecond laser with 780 nm center wavelength were used for neuronal activation and the corresponding neuronal signals were measured by MEAs at the same time. We found that one-photon light via caged glutamate provoked periodic spiking. In contrast, the femtosecond pulse irradiation generated repetitive firing at constant rates. Response times of one-photon and two-photon stimulation were around 200 ms and 50 ms, respectively. We also quantified neural responses, by varying optical parameters such as exposure time and irradiation power. © The Royal Society of Chemistry 2016
URI
https://pr.ibs.re.kr/handle/8788114/2713
ISSN
1473-0197
Appears in Collections:
Center for Soft and Living Matter(첨단연성물질 연구단) > Journal Papers (저널논문)
Files in This Item:
One-photon and two-photon stimulation of neurons in a microfluidic culture system.pdfDownload

qrcode

  • facebook

    twitter

  • Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse