Optogenetic control of endogenous Ca2+ channels in vivo

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Title
Optogenetic control of endogenous Ca2+ channels in vivo
Author(s)
Taeyoon Kyung; Sangkyu Lee; Jung Eun Kim; Taesup Cho; Hyerim Park; Yun-Mi Jeong; Dongkyu Kim; Anna Shin; Sungsoo Kim; Jinhee Baek; Jihoon Kim; Na Yeon Kim; Doyeon Woo; Sujin Chae; Cheol-Hee Kim; Hee-Sup Shin; Yong-Mahn Han; Daesoo Kim; Won Do Heo
Publication Date
2015-10
Journal
NATURE BIOTECHNOLOGY, v.33, no.10, pp.1092 - 1099
Publisher
NATURE PUBLISHING GROUP
Abstract
Calcium (Ca2+) signals that are precisely modulated in space and time mediate a myriad of cellular processes, including contraction, excitation, growth, differentiation and apoptosis1. However, study of Ca2+ responses has been hampered by technological limitations of existing Ca2+-modulating tools. Here we present OptoSTIM1, an optogenetic tool for manipulating intracellular Ca2+ levels through activation of Ca2+-selective endogenous Ca2+ release−activated Ca2+ (CRAC) channels. Using OptoSTIM1, which combines a plant photoreceptor2,3 and the CRAC channel regulator STIM1 (ref. 4), we quantitatively and qualitatively controlled intracellular Ca2+ levels in various biological systems, including zebrafish embryos and human embryonic stem cells. We demonstrate that activating OptoSTIM1 in the CA1 hippocampal region of mice selectively reinforced contextual memory formation. The broad utility of OptoSTIM1 will expand our mechanistic understanding of numerous Ca2+-associated processes and facilitate screening for drug candidates that antagonize Ca2+ signals.
URI
https://pr.ibs.re.kr/handle/8788114/1852
ISSN
1087-0156
Appears in Collections:
Center for Cognition and Sociality(인지 및 사회성 연구단) > Journal Papers (저널논문)
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