Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters

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Title
Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters
Author(s)
Jihyun Lee; Juhee Park; Lee, HH; Park, H; Kim, HI; Won Jong Kim
Publication Date
2015-01
Journal
BIOSENSORS & BIOELECTRONICS, v.68, no.68, pp.642 - 647
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Abstract
A fluorescenceswitchthatconsistsofDNA-templatedsilvernanoclusters(DNA-AgNCs)triggeredby silverion(Agþ) isdevelopedtodetectAgþ. Themechanismofthe fluorescence switchingofDNA-AgNCs is investigatedby fluorescence spectroscopy,circulardichroismspectroscopy,DNAhybridizationassay and massspectrometry.Agþ induces adimericstructureofCyt12-AgNCsbyformingabridgebetween two Cyt12-AgNCs,whereCyt12 is cytosine12-mer;thisdimerformationcausesthe fluorescence change of Cyt12-AgNCs fromredtogreen.UsingthisAgþ-triggered fluorescenceswitch,wesuccessfullydetected Agþ at concentrationsaslowas10nM.Furthermore,wequantitativelydetectedtheAgþ in the Silmazins, whichisdermatologicalburnointmenthavingsilversulfadiazine.Agþ detection usingthis fluorescence switchhashighselectivityandsensitivity,andshortresponsetime,andcanbeusedsuc- cessfully eveninthepresenceofothermetalions.
URI
https://pr.ibs.re.kr/handle/8788114/1636
ISSN
0956-5663
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
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