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Deciphering the Specific High-Affinity Binding of Cucurbit[7]uril to Amino Acids in Water

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Title
Deciphering the Specific High-Affinity Binding of Cucurbit[7]uril to Amino Acids in Water
Author(s)
Lee J.W.; Lee H.H.L.; Young Ho Ko; Kimoon Kim; High I. Kim
Publication Date
2015-04
Journal
JOURNAL OF PHYSICAL CHEMISTRY B, v.119, no.13, pp.4628 - 4636
Publisher
AMER CHEMICAL SOC
Abstract
This work presents a systematic study on the host-guest interactions between the macrocyclic host molecule cucurbit[7]uril (CB[7]) and amino acids (AAs) including three basic AAs (Lys, Arg, and His) and three aromatic AAs (Phe, Tyr, and Trp) to elucidate the origin of the high selectivity of CB[7] toward AA residues in proteins. Complex formation between CB[7] and each AA was examined in solution (by isothermal titration calorimetry and NMR) as well as in the gas phase (by ion mobility mass spectrometry and collision-induced dissociation), and the results were further combined with computational investigations. Generally, the aromatic AAs show higher binding affinities than the basic AAs in buffer solutions with various pH values. On the contrary, the gas-phase stabilities of the basic AA complex ions are higher than those of the aromatic AA complex ions, suggesting that the direct ion-dipole interactions between the charged side chains of the basic AAs and the polar carbonyl groups of CB[7] predominate in the absence of water. The ion-dipole interactions are less significant in water, since the original interactions of the guests with water are lost upon complex formation. In contrast, the transfer of the hydrophobic groups from the bulk into the hydrophobic CB[7] cavity suffers less from the desolvation penalty, resulting in higher binding affinities in water. Therefore, initial guest solvation is another key factor which should be considered when designing high-affinity host-guest systems, in addition to the contribution from the release of high-energy water molecules from the CB[7] cavity (J. Am. Chem. Soc. 2012, 134, 15318-15323). (Chemical Equation Presented). © 2015 American Chemical Society
URI
https://pr.ibs.re.kr/handle/8788114/1627
ISSN
1520-6106
Appears in Collections:
Center for Self-assembly and Complexity(복잡계 자기조립 연구단) > Journal Papers (저널논문)
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