IBS Publications Repository: Reversible protein inactivation by optogenetic trapping in cells

heo,wondo's photo.

Reversible protein inactivation by optogenetic trapping in cells

Cited 115 time in webofscience

Cited 121 time in

1,669 Viewed 304 Downloaded

Author(s): Sangkyu Lee; Hyerim Park; Taeyoon Kyung; Na Yeon Kim; Sungsoo Kim; Jihoon Kim; Won Do Heo

Subject: calcium calmodulin dependent protein kinase II, ; cryptochrome 2, ; epitope, ; nanobody, ; phosphatidylinositol 3 kinase, ; Arabidopsis thaliana, ; article, ; cell function, ; cell polarity, ; cell structure, ; cell viability, ; cytoskeleton, ; endosome, ; HeLa cell line, ; human, ; human cell, ; light activated reversible inhibition by assembled trap, ; mammal cell, ; microtubule, ; nonhuman, ; optogenetics, ; priority journal, ; protein expression, ; protein function, ; protein processing, ; reversible protein inactivation, ; signal transduction

Abstract: We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP. © 2014 Nature America, Inc.