IBS Publications Repository: Reversible protein inactivation by optogenetic trapping in cells

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Reversible protein inactivation by optogenetic trapping in cells

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Author(s): Sangkyu Lee; Hyerim Park; Taeyoon Kyung; Na Yeon Kim; Sungsoo Kim; Jihoon Kim; Won Do Heo

Subject: calcium calmodulin dependent protein kinase II, ; cryptochrome 2, ; epitope, ; nanobody, ; phosphatidylinositol 3 kinase, ; Arabidopsis thaliana, ; article, ; cell function, ; cell polarity, ; cell structure, ; cell viability, ; cytoskeleton, ; endosome, ; HeLa cell line, ; human, ; human cell, ; light activated reversible inhibition by assembled trap, ; mammal cell, ; microtubule, ; nonhuman, ; optogenetics, ; priority journal, ; protein expression, ; protein function, ; protein processing, ; reversible protein inactivation, ; signal transduction

Abstract: We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP. © 2014 Nature America, Inc.